Effects of morphology and pore size of mesoporous silicas on the efficiency of an immobilized enzyme

Ping Chung Kuo, Zhi Xun Lin, Tzi Yi Wu, Chun Han Hsu, Hong Ping Lin, Tian Shung Wu

Research output: Contribution to journalArticlepeer-review

Abstract

An investigation is performed into the efficiency of theStreptomyces griseusHUT 6037 enzyme immobilized in three different mesoporous silicas, namely mesoporous silica film, mesocellular foam, and rod-like SBA-15. It is shown that for all three supports, the pH value changes the surface charge and charge density and hence determines the maximum loading capacity of the enzyme. The products of the enzyme hydrolytic reaction are analyzed by1H-NMR. The results show that among the three silica supports, the mesoporous silica film (with a channel length in the range of 60-100 nm) maximizes the accessibility of the immobilized enzyme. The loading capacity of the enzyme is up to 95% at pH 7 and the activity of the immobilized enzyme is maintained for more than 15 days when using a silica film support. The order of the activity of the enzyme immobilized in different mesoporous silica supports is: mesoporous silica film > mesocellular foam > rod-like SBA-15. Furthermore, the immobilized enzyme can be easily separated from the reaction solutionviasimple filtration or centrifugation methods and re-used for hydrolytic reaction as required.

Original languageEnglish
Pages (from-to)10010-10017
Number of pages8
JournalRSC Advances
Volume11
Issue number17
DOIs
Publication statusPublished - 2021 Mar 2

All Science Journal Classification (ASJC) codes

  • Chemistry(all)
  • Chemical Engineering(all)

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