TY - JOUR
T1 - Effects of nitric oxide on human spermatozoa activity, fertilization and mouse embryonic development
AU - Wu, T. P.
AU - Huang, B. M.
AU - Tsai, H. C.
AU - Lui, M. C.
AU - Liu, M. Y.
PY - 2004/1/1
Y1 - 2004/1/1
N2 - This study was conducted to investigate the effects of nitric oxide (NO) on human sperm activity, human sperm-oocyte fusion and mouse embryonic development. Results showed that various concentrations of NO synthase blocker, Nω-nitro-L-arginine methyl ester, did not affect sperm cell motility at 0, 1, 2 or 4 hr, respectively. In contrast, sodium nitroprusside (SNP) significantly inhibited sperm cell motility and caused apoptosis. The adversely dose-dependent effect was only observed if SNP was freshly prepared. Adenosine triphosphate reversed the hazardous effect of SNP on sperm activity/viability. Hemoglobin neutralized the adverse effect of SNP. In hemi-zona sperm fusion test, the number of sperm bound to the zona in the presence of 10-4 M SNP was significantly less than the control group. SNP at 10-4 M caused all mouse embryonic development arrest. 46% and 56% of zygote reached the blastocyst stage with the treatment of 10-6 M and 10-8 M SNP, respectively, while the control reached 70%. NO adversely affected human sperm activity, human sperm-zona binding and embryonic development. It would appear that high concentration of NO may potentially decrease fertility.
AB - This study was conducted to investigate the effects of nitric oxide (NO) on human sperm activity, human sperm-oocyte fusion and mouse embryonic development. Results showed that various concentrations of NO synthase blocker, Nω-nitro-L-arginine methyl ester, did not affect sperm cell motility at 0, 1, 2 or 4 hr, respectively. In contrast, sodium nitroprusside (SNP) significantly inhibited sperm cell motility and caused apoptosis. The adversely dose-dependent effect was only observed if SNP was freshly prepared. Adenosine triphosphate reversed the hazardous effect of SNP on sperm activity/viability. Hemoglobin neutralized the adverse effect of SNP. In hemi-zona sperm fusion test, the number of sperm bound to the zona in the presence of 10-4 M SNP was significantly less than the control group. SNP at 10-4 M caused all mouse embryonic development arrest. 46% and 56% of zygote reached the blastocyst stage with the treatment of 10-6 M and 10-8 M SNP, respectively, while the control reached 70%. NO adversely affected human sperm activity, human sperm-zona binding and embryonic development. It would appear that high concentration of NO may potentially decrease fertility.
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U2 - 10.1080/01485010490455494
DO - 10.1080/01485010490455494
M3 - Article
C2 - 15204684
AN - SCOPUS:80052494577
VL - 50
SP - 173
EP - 179
JO - Systems Biology in Reproductive Medicine
JF - Systems Biology in Reproductive Medicine
SN - 1939-6368
IS - 3
ER -