Effects of pH and fermentation strategies on 2,3-butanediol production with an isolated Klebsiella sp. Zmd30 strain

Chiao Ling Wong, Hong Wei Yen, Chung Liang Lin, Jo-Shu Chang

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

This study examined the effects of pH-controlled and fermentation strategies on 2,3-butanediol (2,3-BDO) production by an isolated Klebsiella sp. Zmd30 strain. The pH value of 6.0 was found to be optimal among the investigated range of 4.5-9.0. In batch fermentation, the concentration, productivity and yield of 2,3-BDO were 57.17. g/l, 1.59. g/l/h and 82%, respectively. The 2,3-BDO production by Klebsiella sp. Zmd30 was found to be growth-associated. Higher 2,3-BDO concentration (110. g/l) and yield (94%) was obtained by using fed-batch operation, but the productivity was lower (0.88. g/l/h) as compared to that when using batch operation. The highest 2,3-BDO productivity of 2.81. g/l/h was obtained with the continuous culture at a hydraulic retention time (HRT) of 12. h. The results suggest that fed-batch operation might be most suitable for commercialized 2,3-BDO production, due to obtaining a high concentration and yield.

Original languageEnglish
Pages (from-to)169-176
Number of pages8
JournalBioresource Technology
Volume152
DOIs
Publication statusPublished - 2014 Jan 1

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Fermentation
fermentation
Productivity
productivity
Hydraulics
hydraulics
effect
2,3-butylene glycol
pH-value

All Science Journal Classification (ASJC) codes

  • Bioengineering
  • Environmental Engineering
  • Renewable Energy, Sustainability and the Environment
  • Waste Management and Disposal

Cite this

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abstract = "This study examined the effects of pH-controlled and fermentation strategies on 2,3-butanediol (2,3-BDO) production by an isolated Klebsiella sp. Zmd30 strain. The pH value of 6.0 was found to be optimal among the investigated range of 4.5-9.0. In batch fermentation, the concentration, productivity and yield of 2,3-BDO were 57.17. g/l, 1.59. g/l/h and 82{\%}, respectively. The 2,3-BDO production by Klebsiella sp. Zmd30 was found to be growth-associated. Higher 2,3-BDO concentration (110. g/l) and yield (94{\%}) was obtained by using fed-batch operation, but the productivity was lower (0.88. g/l/h) as compared to that when using batch operation. The highest 2,3-BDO productivity of 2.81. g/l/h was obtained with the continuous culture at a hydraulic retention time (HRT) of 12. h. The results suggest that fed-batch operation might be most suitable for commercialized 2,3-BDO production, due to obtaining a high concentration and yield.",
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Effects of pH and fermentation strategies on 2,3-butanediol production with an isolated Klebsiella sp. Zmd30 strain. / Wong, Chiao Ling; Yen, Hong Wei; Lin, Chung Liang; Chang, Jo-Shu.

In: Bioresource Technology, Vol. 152, 01.01.2014, p. 169-176.

Research output: Contribution to journalArticle

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