TY - JOUR
T1 - Effects of pH and fermentation strategies on 2,3-butanediol production with an isolated Klebsiella sp. Zmd30 strain
AU - Wong, Chiao Ling
AU - Yen, Hong Wei
AU - Lin, Chung Liang
AU - Chang, Jo Shu
N1 - Funding Information:
The authors gratefully acknowledge the financial support this study received from Taiwan’s National Science Council under Grant Numbers 102-3113-P-006-016 and 101-2221-E-006-094- MY3. The support received from the Top University grant (also known as “5-year-50-billion” grant) from Taiwan’s Ministry of Education is also appreciated.
PY - 2014/1
Y1 - 2014/1
N2 - This study examined the effects of pH-controlled and fermentation strategies on 2,3-butanediol (2,3-BDO) production by an isolated Klebsiella sp. Zmd30 strain. The pH value of 6.0 was found to be optimal among the investigated range of 4.5-9.0. In batch fermentation, the concentration, productivity and yield of 2,3-BDO were 57.17. g/l, 1.59. g/l/h and 82%, respectively. The 2,3-BDO production by Klebsiella sp. Zmd30 was found to be growth-associated. Higher 2,3-BDO concentration (110. g/l) and yield (94%) was obtained by using fed-batch operation, but the productivity was lower (0.88. g/l/h) as compared to that when using batch operation. The highest 2,3-BDO productivity of 2.81. g/l/h was obtained with the continuous culture at a hydraulic retention time (HRT) of 12. h. The results suggest that fed-batch operation might be most suitable for commercialized 2,3-BDO production, due to obtaining a high concentration and yield.
AB - This study examined the effects of pH-controlled and fermentation strategies on 2,3-butanediol (2,3-BDO) production by an isolated Klebsiella sp. Zmd30 strain. The pH value of 6.0 was found to be optimal among the investigated range of 4.5-9.0. In batch fermentation, the concentration, productivity and yield of 2,3-BDO were 57.17. g/l, 1.59. g/l/h and 82%, respectively. The 2,3-BDO production by Klebsiella sp. Zmd30 was found to be growth-associated. Higher 2,3-BDO concentration (110. g/l) and yield (94%) was obtained by using fed-batch operation, but the productivity was lower (0.88. g/l/h) as compared to that when using batch operation. The highest 2,3-BDO productivity of 2.81. g/l/h was obtained with the continuous culture at a hydraulic retention time (HRT) of 12. h. The results suggest that fed-batch operation might be most suitable for commercialized 2,3-BDO production, due to obtaining a high concentration and yield.
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U2 - 10.1016/j.biortech.2013.10.101
DO - 10.1016/j.biortech.2013.10.101
M3 - Article
C2 - 24291317
AN - SCOPUS:84888411458
SN - 0960-8524
VL - 152
SP - 169
EP - 176
JO - Bioresource technology
JF - Bioresource technology
ER -