Efficient and stable enzyme immobilization in a block copolypeptide vesicle-templated biomimetic silica support

Jun Kun Lai, Tzu Han Chuang, Jeng Shiung Jan, Steven Sheng Shih Wang

Research output: Contribution to journalArticlepeer-review

33 Citations (Scopus)

Abstract

We report the immobilization of a model enzyme, papain, within silica matrices by combining vesiclization of poly-l-lysine-b-polyglycine block copolypeptides with following silica mineralization. Our novel strategy utilizes block polypeptide vesicles to induce the condensation of orthosilicic acid while trapping an enzyme within and between vesicles. The polypeptide mediated silica-immobilized enzyme exhibits enhanced pH and thermal stability and reusability, comparing with the free and vesicle encapsulated enzyme. The enhanced enzymatic activity in the immobilized enzyme is due to the confinement of the enzyme in the polypeptide mediated silica matrices. Kinetic analysis shows that the enzyme functionality is determined by the structure and property of silica/polypeptide matrices. The proposed novel strategy provides an alternative route for the synthesis of a broad range of functional bionanocomposites entrapped within silica nanostructures.

Original languageEnglish
Pages (from-to)51-58
Number of pages8
JournalColloids and Surfaces B: Biointerfaces
Volume80
Issue number1
DOIs
Publication statusPublished - 2010 Oct 1

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Surfaces and Interfaces
  • Physical and Theoretical Chemistry
  • Colloid and Surface Chemistry

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