Efficient carbon dioxide sequestration by using recombinant carbonic anhydrase

Shih I. Tan, Yin Lung Han, You Jin Yu, Chen Yaw Chiu, Yu Kaung Chang, Shoung Ouyang, Kai Chun Fan, Kuei Ho Lo, I. Son Ng

Research output: Contribution to journalArticlepeer-review

16 Citations (Scopus)

Abstract

Efficient biological carbon dioxide sequestration for slowing down the climate changes requires a highly active and stable carbonic anhydrase (CA). A CA from Mesorhizobium loti (i.e., MlCA) was first over-expressed in E. coli via different vectors and a concentration of up to 5 g/L was obtained with pET32a(+). The whole cell biocatalyst was stable between pH 4.0 and pH 9.0 and maintained 82% activity within 6% salinity. The cations NH4 +, K+, Na+, Mg2+, Ca2+ and Mn2+ did not affect the catalytic activity, while Co2+, Cu2+, and Zn2+ inhibited >70% biocatalyst activity at 5 mM. The Kcat and Km of whole cell CA was observed to be 1.76 × 106 /s and 0.0133 mM, while that of the crude enzyme was of 2.35 × 106 s−1 and 0.0364 mM, respectively. The effective diffusivity coefficient (De) of whole cell CA was 3.98 μ m2/s. Whole cell biocatalyst immobilized in 2% (w/v) agar could be reused for 6 times for CO2 sequestration and remained stable for 40 days. A novel two-column system with immobilized whole cell biocatalyst and recombinant MlCA effectively converted 100% CO2 to CaCO3 within 3.5 min. This provides an alternative, eco-friendly and low-cost process for carbon capture and storage (CCS) in the future.

Original languageEnglish
Pages (from-to)38-46
Number of pages9
JournalProcess Biochemistry
Volume73
DOIs
Publication statusPublished - 2018 Oct

All Science Journal Classification (ASJC) codes

  • Bioengineering
  • Biochemistry
  • Applied Microbiology and Biotechnology

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