TY - JOUR
T1 - Efficient fucoidan extraction and purification from Sargassum cristaefolium and preclinical dermal biological activity assessments of the purified fucoidans
AU - Lin, En Ting
AU - Lee, Yi Chieh
AU - Wang, Hui Min David
AU - Chiu, Chen Yaw
AU - Chang, Yu Kaung
AU - Huang, Chun Yung
AU - Chang, Chia Che
AU - Tsai, Pei Chien
AU - Chang, Jo Shu
N1 - Funding Information:
This research was supported by the Ministry of Science and Technology (MOST), Taiwan under grant nos. MOST 110-2221-E-992-011, 110-2221-E-005-010, 109-2221-E-029-004, 110-2221-E-029-005, 110-3116-F-006 -003, 110-2221-E-029 -004 -MY3, 110-2621-M-029 -001, and 109-2622-E-110 -011. The authors also thank Kaohsiung Armed Forces General Hospital (KAFGH_D_109014), National Chung Hsing University (ENABLE: 108ST001B and 110ST001G), Kasetsart University Joint Research Project (108RA129A), and NCHU-MIRDC Bilateral Joint Research Project (110S0703B).
Funding Information:
This research was supported by the Ministry of Science and Technology (MOST), Taiwan under grant nos. MOST 110-2221-E-992-011, 110-2221-E-005-010, 109-2221-E-029-004, 110-2221-E-029-005, 110-3116-F-006 -003, 110-2221-E-029 -004 -MY3, 110-2621-M-029 -001, and 109-2622-E-110 -011. The authors also thank Kaohsiung Armed Forces General Hospital (KAFGH_D_109014), National Chung Hsing University (ENABLE: 108ST001B and 110ST001G), Kasetsart University Joint Research Project (108RA129A), and NCHU-MIRDC Bilateral Joint Research Project (110S0703B).
Publisher Copyright:
© 2022
PY - 2022/8
Y1 - 2022/8
N2 - Background: Fucoidan is a heterologous polysaccharide consisting mainly of L-fucose with sulfuric acid functional groups, and it is considered to be a repository of bioactive compounds with excellent therapeutic and medical potential, such as antioxidant, anti-inflammatory, antiangiogenic, antitumor, and antibacterial capabilities. Methods: Subcritical water extraction was used to extract fucoidan from Sargassum. Fucoidans with different molecular weight (LMW) fractions were obtained by H2O2 hydrolysis, and the effect of LMW on their bio-functions was investigated. Significant Findings: Metal chelating, iron-reducing and free radical scavenging properties of purified fucoidans demonstrated outstanding anti-oxidative capacities of SWE fractions. In cellular viability assay, SWE was not inhibitory towards RAW 264.7, HaCaT, and Hs68 cells, even when high dosages were used. Anti-inflammatory activity was brought about by SWE administration after UVB exposure, demonstrating that the injured skin tissue was repaired and collagen was regenerated, as shown by in vitro and in vivo tests.
AB - Background: Fucoidan is a heterologous polysaccharide consisting mainly of L-fucose with sulfuric acid functional groups, and it is considered to be a repository of bioactive compounds with excellent therapeutic and medical potential, such as antioxidant, anti-inflammatory, antiangiogenic, antitumor, and antibacterial capabilities. Methods: Subcritical water extraction was used to extract fucoidan from Sargassum. Fucoidans with different molecular weight (LMW) fractions were obtained by H2O2 hydrolysis, and the effect of LMW on their bio-functions was investigated. Significant Findings: Metal chelating, iron-reducing and free radical scavenging properties of purified fucoidans demonstrated outstanding anti-oxidative capacities of SWE fractions. In cellular viability assay, SWE was not inhibitory towards RAW 264.7, HaCaT, and Hs68 cells, even when high dosages were used. Anti-inflammatory activity was brought about by SWE administration after UVB exposure, demonstrating that the injured skin tissue was repaired and collagen was regenerated, as shown by in vitro and in vivo tests.
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U2 - 10.1016/j.jtice.2022.104294
DO - 10.1016/j.jtice.2022.104294
M3 - Article
AN - SCOPUS:85126301979
VL - 137
JO - Journal of the Taiwan Institute of Chemical Engineers
JF - Journal of the Taiwan Institute of Chemical Engineers
SN - 1876-1070
M1 - 104294
ER -