Efficient reactivation of latent herpes simplex virus from mouse central nervous system tissues

Shih Heng Chen, Hui Wen Yao, Wen Yen Huang, Kuei Sen Hsu, Huan Yao Lei, Ai Li Shiau, Shun Hua Chen

Research output: Contribution to journalArticlepeer-review

28 Citations (Scopus)

Abstract

For decades, numerous ex vivo studies have documented that latent herpes simplex virus (HSV) reactivates efficiently from ganglia, but rarely from the central nervous systems (CNS), of mice when assayed by mincing tissues before expiant culture, despite the presence of viral genomes in both sites. Here we show that 88% of mouse brain stems reactivated latent virus when they were dissociated into cell suspensions before ex vivo explant culture. The efficient reactivation of HSV from the mouse CNS was demonstrated with more than one viral strain, viral serotype, and mouse strain, further indicating that the CNS can be an authentic latency site for HSV with the potential to cause recurrent disease.

Original languageEnglish
Pages (from-to)12387-12392
Number of pages6
JournalJournal of Virology
Volume80
Issue number24
DOIs
Publication statusPublished - 2006 Dec

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Immunology
  • Insect Science
  • Virology

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