Efficient 1H nuclear magnetic resonance method for improved quality control analyses of Ginkgo constituents

Chia Ying Li; Chun Hua Lin; Chia Che Wu; Kuo Hsiung Lee; Tian Shung Wu

doi:10.1021/jf049920h

Efficient ¹H nuclear magnetic resonance method for improved quality control analyses of Ginkgo constituents

Chia Ying Li, Chun Hua Lin, Chia Che Wu, Kuo Hsiung Lee, Tian Shung Wu

School of Pharmacy

Research output: Contribution to journal › Article › peer-review

46 Citations (Scopus)

Abstract

We developed an analytical method using ¹H nuclear magnetic resonance (NMR) spectrometry to resolve analytical problems with Ginkgo. After a simple hydrolysis step, an NMR analysis of the terpene trilactone H-12 signals and the flavonol aglycone H-2′ (or H-2′/6′ for kaempferol) signals was performed. By comparing the solvent effects on the resolution of these signals, methanol-d₄-benzene-d₆ (65:35) was selected as the optimal ¹H NMR solvent. The amounts of terpene lactones and flavonol aglycones in various commercial Ginkgo products and Ginkgo leaves were determined. This newly developed ¹H NMR method enables the simultaneous analysis of terpene trilactones and flavonols and allows simple, rapid quantification of these compounds in pharmaceutical Ginkgo preparations.

Original language	English
Pages (from-to)	3721-3725
Number of pages	5
Journal	Journal of Agricultural and Food Chemistry
Volume	52
Issue number	12
DOIs	https://doi.org/10.1021/jf049920h
Publication status	Published - 2004 Jun 16

All Science Journal Classification (ASJC) codes

General Chemistry
General Agricultural and Biological Sciences

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10.1021/jf049920h

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title = "Efficient 1H nuclear magnetic resonance method for improved quality control analyses of Ginkgo constituents",

abstract = "We developed an analytical method using 1H nuclear magnetic resonance (NMR) spectrometry to resolve analytical problems with Ginkgo. After a simple hydrolysis step, an NMR analysis of the terpene trilactone H-12 signals and the flavonol aglycone H-2′ (or H-2′/6′ for kaempferol) signals was performed. By comparing the solvent effects on the resolution of these signals, methanol-d4-benzene-d6 (65:35) was selected as the optimal 1H NMR solvent. The amounts of terpene lactones and flavonol aglycones in various commercial Ginkgo products and Ginkgo leaves were determined. This newly developed 1H NMR method enables the simultaneous analysis of terpene trilactones and flavonols and allows simple, rapid quantification of these compounds in pharmaceutical Ginkgo preparations.",

author = "Li, {Chia Ying} and Lin, {Chun Hua} and Wu, {Chia Che} and Lee, {Kuo Hsiung} and Wu, {Tian Shung}",

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T1 - Efficient 1H nuclear magnetic resonance method for improved quality control analyses of Ginkgo constituents

AU - Li, Chia Ying

AU - Lin, Chun Hua

AU - Wu, Chia Che

AU - Lee, Kuo Hsiung

AU - Wu, Tian Shung

PY - 2004/6/16

Y1 - 2004/6/16

N2 - We developed an analytical method using 1H nuclear magnetic resonance (NMR) spectrometry to resolve analytical problems with Ginkgo. After a simple hydrolysis step, an NMR analysis of the terpene trilactone H-12 signals and the flavonol aglycone H-2′ (or H-2′/6′ for kaempferol) signals was performed. By comparing the solvent effects on the resolution of these signals, methanol-d4-benzene-d6 (65:35) was selected as the optimal 1H NMR solvent. The amounts of terpene lactones and flavonol aglycones in various commercial Ginkgo products and Ginkgo leaves were determined. This newly developed 1H NMR method enables the simultaneous analysis of terpene trilactones and flavonols and allows simple, rapid quantification of these compounds in pharmaceutical Ginkgo preparations.

AB - We developed an analytical method using 1H nuclear magnetic resonance (NMR) spectrometry to resolve analytical problems with Ginkgo. After a simple hydrolysis step, an NMR analysis of the terpene trilactone H-12 signals and the flavonol aglycone H-2′ (or H-2′/6′ for kaempferol) signals was performed. By comparing the solvent effects on the resolution of these signals, methanol-d4-benzene-d6 (65:35) was selected as the optimal 1H NMR solvent. The amounts of terpene lactones and flavonol aglycones in various commercial Ginkgo products and Ginkgo leaves were determined. This newly developed 1H NMR method enables the simultaneous analysis of terpene trilactones and flavonols and allows simple, rapid quantification of these compounds in pharmaceutical Ginkgo preparations.

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Efficient ¹H nuclear magnetic resonance method for improved quality control analyses of Ginkgo constituents

Abstract

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