Efficient 1H nuclear magnetic resonance method for improved quality control analyses of Ginkgo constituents

Chia Ying Li, Chun Hua Lin, Chia Che Wu, Kuo Hsiung Lee, Tian-Shung Wu

Research output: Contribution to journalArticle

39 Citations (Scopus)

Abstract

We developed an analytical method using 1H nuclear magnetic resonance (NMR) spectrometry to resolve analytical problems with Ginkgo. After a simple hydrolysis step, an NMR analysis of the terpene trilactone H-12 signals and the flavonol aglycone H-2′ (or H-2′/6′ for kaempferol) signals was performed. By comparing the solvent effects on the resolution of these signals, methanol-d4-benzene-d6 (65:35) was selected as the optimal 1H NMR solvent. The amounts of terpene lactones and flavonol aglycones in various commercial Ginkgo products and Ginkgo leaves were determined. This newly developed 1H NMR method enables the simultaneous analysis of terpene trilactones and flavonols and allows simple, rapid quantification of these compounds in pharmaceutical Ginkgo preparations.

Original languageEnglish
Pages (from-to)3721-3725
Number of pages5
JournalJournal of Agricultural and Food Chemistry
Volume52
Issue number12
DOIs
Publication statusPublished - 2004 Jun 16

Fingerprint

Ginkgo
Ginkgo biloba
Quality Control
quality control
Quality control
nuclear magnetic resonance spectroscopy
Terpenes
Magnetic Resonance Spectroscopy
flavonols
Nuclear magnetic resonance
terpenoids
Flavonols
benzene
Lactones
kaempferol
Benzene
lactones
methodology
Spectrometry
analytical methods

All Science Journal Classification (ASJC) codes

  • Agricultural and Biological Sciences (miscellaneous)
  • Food Science
  • Chemistry (miscellaneous)

Cite this

Li, Chia Ying ; Lin, Chun Hua ; Wu, Chia Che ; Lee, Kuo Hsiung ; Wu, Tian-Shung. / Efficient 1H nuclear magnetic resonance method for improved quality control analyses of Ginkgo constituents. In: Journal of Agricultural and Food Chemistry. 2004 ; Vol. 52, No. 12. pp. 3721-3725.
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Efficient 1H nuclear magnetic resonance method for improved quality control analyses of Ginkgo constituents. / Li, Chia Ying; Lin, Chun Hua; Wu, Chia Che; Lee, Kuo Hsiung; Wu, Tian-Shung.

In: Journal of Agricultural and Food Chemistry, Vol. 52, No. 12, 16.06.2004, p. 3721-3725.

Research output: Contribution to journalArticle

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AB - We developed an analytical method using 1H nuclear magnetic resonance (NMR) spectrometry to resolve analytical problems with Ginkgo. After a simple hydrolysis step, an NMR analysis of the terpene trilactone H-12 signals and the flavonol aglycone H-2′ (or H-2′/6′ for kaempferol) signals was performed. By comparing the solvent effects on the resolution of these signals, methanol-d4-benzene-d6 (65:35) was selected as the optimal 1H NMR solvent. The amounts of terpene lactones and flavonol aglycones in various commercial Ginkgo products and Ginkgo leaves were determined. This newly developed 1H NMR method enables the simultaneous analysis of terpene trilactones and flavonols and allows simple, rapid quantification of these compounds in pharmaceutical Ginkgo preparations.

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