TY - JOUR
T1 - Eicosapentaenoic acid production from Nannochloropsis oceanica CY2 using deep sea water in outdoor plastic-bag type photobioreactors
AU - Chen, Chun Yen
AU - Nagarajan, Dillirani
AU - Cheah, Wai Yan
N1 - Funding Information:
The authors gratefully acknowledge financial support from Taiwan’s National Science Council under grant number MOST 106-3113-E-006-011 , MOST 106-3113-E-006 -004 -CC2 and MOST 106-3114-E-006-008 .
Publisher Copyright:
© 2017 Elsevier Ltd
PY - 2018/4
Y1 - 2018/4
N2 - In this study, Nannochloropsis oceanica CY2 was grown in deep-sea water (DSW)-based medium in 5-L plastic bag-type photobioreactors (PBRs) for the autotrophic production of Eicosapentaenoic acid (EPA, 20:5n-3). EPA production of N. oceanica CY2 was stimulated when it was grown in 100% DSW amended with 1.5 g L−1 NaNO3, achieving a EPA content of 3.1% and a biomass concentration of 3.3 g L−1. An outdoor-simulated microalgae cultivation system was also conducted to validate the feasibility of outdoor cultivation of the CY2 strain in plastic bag-type PBRs. Using an inoculum size of 0.6 g/L, the biomass concentration in the PBR culture was 3.5 g L−1, while the EPA content and productivity reached a maximal level of 4.12% and 7.49 mg L−1 d−1, respectively. When the PBRs were operated on semi-batch mode, the EPA productivity could further increase to 9.9 mg L−1 d−1 with a stable EPA content of 4.1%.
AB - In this study, Nannochloropsis oceanica CY2 was grown in deep-sea water (DSW)-based medium in 5-L plastic bag-type photobioreactors (PBRs) for the autotrophic production of Eicosapentaenoic acid (EPA, 20:5n-3). EPA production of N. oceanica CY2 was stimulated when it was grown in 100% DSW amended with 1.5 g L−1 NaNO3, achieving a EPA content of 3.1% and a biomass concentration of 3.3 g L−1. An outdoor-simulated microalgae cultivation system was also conducted to validate the feasibility of outdoor cultivation of the CY2 strain in plastic bag-type PBRs. Using an inoculum size of 0.6 g/L, the biomass concentration in the PBR culture was 3.5 g L−1, while the EPA content and productivity reached a maximal level of 4.12% and 7.49 mg L−1 d−1, respectively. When the PBRs were operated on semi-batch mode, the EPA productivity could further increase to 9.9 mg L−1 d−1 with a stable EPA content of 4.1%.
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U2 - 10.1016/j.biortech.2017.12.102
DO - 10.1016/j.biortech.2017.12.102
M3 - Article
C2 - 29328929
AN - SCOPUS:85044350984
SN - 0960-8524
VL - 253
SP - 1
EP - 7
JO - Bioresource technology
JF - Bioresource technology
ER -
