Electrochemical evaluation of avidin-biotin interaction on self-assembled gold electrodes

Shinn Jyh Ding, Bin Wha Chang, Ching Chou Wu, Min Feng Lai, Hsien-Chang Chang

Research output: Contribution to journalArticle

57 Citations (Scopus)

Abstract

The avidin-biotin interaction on 11-mercaptoundecanoic acid self-assembled gold electrodes was investigated by means of cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS). The interfacial properties of the modified electrodes were evaluated in the presence of the Fe(CN) 6 3-/4- couple redox as a probe. A simple equivalent circuit model with a constant phase element was used to interpret the obtained impedance spectra. The results of cyclic voltammetry showed that the voltammetric behavior of the redox probe was influenced by the electrode surface modification. It is evident that the accumulation of treated substances and the binding of biotin to avidin on the electrode surface resulted in the increasing electron-transfer resistance and the decreasing capacitance. The changes in the electron-transfer resistance on the avidin-modified electrodes were more sensitive than that in the capacitance while detecting biotin over the 2-10 μg/mL concentration. The detection amount can be as low as 20 ng/mL based on the electron-transfer resistance that presented the change of 4.3 kΩ without the use of labels. The development of a rapid, facile, and sensitive method for the quantitation of nanogram quantities of biomolecules utilizing EIS may be achieved.

Original languageEnglish
Pages (from-to)3660-3666
Number of pages7
JournalElectrochimica Acta
Volume50
Issue number18
DOIs
Publication statusPublished - 2005 Jun 10

Fingerprint

Avidin
Biotin
Gold
Electrodes
Electrochemical impedance spectroscopy
Cyclic voltammetry
Electrons
Capacitance
Biomolecules
Equivalent circuits
Surface treatment
Labels
Acids

All Science Journal Classification (ASJC) codes

  • Chemical Engineering(all)
  • Analytical Chemistry
  • Electrochemistry

Cite this

Ding, Shinn Jyh ; Chang, Bin Wha ; Wu, Ching Chou ; Lai, Min Feng ; Chang, Hsien-Chang. / Electrochemical evaluation of avidin-biotin interaction on self-assembled gold electrodes. In: Electrochimica Acta. 2005 ; Vol. 50, No. 18. pp. 3660-3666.
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Electrochemical evaluation of avidin-biotin interaction on self-assembled gold electrodes. / Ding, Shinn Jyh; Chang, Bin Wha; Wu, Ching Chou; Lai, Min Feng; Chang, Hsien-Chang.

In: Electrochimica Acta, Vol. 50, No. 18, 10.06.2005, p. 3660-3666.

Research output: Contribution to journalArticle

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