Electrokinetics-Based Microfluidic Technology for the Rapid Separation and Concentration of Bacteria/Cells/Biomolecules

I. Fang Cheng, Tzu Ying Chen, Hsien Chang Chang

Research output: Chapter in Book/Report/Conference proceedingConference contribution

5 Citations (Scopus)


Conventional techniques for detection of bacteria/cell and assessment of cancer cell typically use DNA techniques, Western blot and ELISA kits that are high cost, complicated processes and long time consuming. Our researches focus on rapid, portable, simple and highly sensitive separation and detection of cells/bacteria/biomolecules for field-use diagnosis. An ideal portable biosensor (molecular or whole cells detections) unit must have several important features: rapid detection time (<10 minutes), high sensitivity (pM level for molecular detection, 103 cells/ml for whole cell detection), high specificity, small and inexpensive instrumentation configuration. Electrochemical impedance/conductance sensing is preferred over optical detection because of cost and portability concerns. Cancer cell detection using heterogeneous medical samples require continuous isolation, sorting, and trapping of the target bioparticles and immunocolloids within a diagnostic chip. We have developed several electrokinetic strategies to rapid separation, concentration and detection of cells/bacteria/biomolecules in a microfluidic chip using such as dielectrophoresis (DEP), traveling-wave dielectrophoresis (twDEP) and electrohydrodynamics (EHD). Several key techniques we done, which on a rapid/simple/label-free detection platform for the highly sensitive on-chip separation/identification/quantification will be introduced in this paper.

Original languageEnglish
Title of host publicationKey Engineering Materials - Development and Application
PublisherTrans Tech Publications
Number of pages5
ISBN (Print)9783038350668
Publication statusPublished - 2014
Event4th International Conference on Key Engineering Materials, ICKEM 2014 - Bali, Indonesia
Duration: 2014 Mar 222014 Mar 23

Publication series

NameAdvanced Materials Research
ISSN (Print)1022-6680


Other4th International Conference on Key Engineering Materials, ICKEM 2014

All Science Journal Classification (ASJC) codes

  • Engineering(all)


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