TY - JOUR
T1 - Electrooxidation of NADH with Co(phen)33+ by NADH dehydrogenase purified from Bacillus stearothermophilus
AU - Matsue, Tomokazu
AU - Yamada, Hiroshi
AU - Chang, Hsien Chang
AU - Uchida, Isamu
N1 - Funding Information:
The present study was partly supported by Asahi Glass Foundation and Grants-in-Aid for Scientific Research (Nos. 01470076 and 0185175) from the Ministry of Education and Culture, Japan.
PY - 1990/12
Y1 - 1990/12
N2 - The NADH dehydrogenase purified from Bacillus stearothermophilus catalyzes effectively the electrochemical oxidation of NADH mediated by Co(phen)3+3. Stopped-flow measurements indicate that the rate constant for the oxidation of the reduced enzyme by Co(phen)3+3 is 5 × 106 M-1 s-1 and that for the oxidation of NADH by the oxidized enzyme > 3 × 107 M-1 s-1. The voltammograms, showing catalytic prepeaks, were simulated using the above rate constants.
AB - The NADH dehydrogenase purified from Bacillus stearothermophilus catalyzes effectively the electrochemical oxidation of NADH mediated by Co(phen)3+3. Stopped-flow measurements indicate that the rate constant for the oxidation of the reduced enzyme by Co(phen)3+3 is 5 × 106 M-1 s-1 and that for the oxidation of NADH by the oxidized enzyme > 3 × 107 M-1 s-1. The voltammograms, showing catalytic prepeaks, were simulated using the above rate constants.
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U2 - 10.1016/0302-4598(90)80034-G
DO - 10.1016/0302-4598(90)80034-G
M3 - Article
AN - SCOPUS:0001759321
SN - 0302-4598
VL - 24
SP - 347
EP - 354
JO - Bioelectrochemistry and Bioenergetics
JF - Bioelectrochemistry and Bioenergetics
IS - 3
ER -