Electrooxidation of NADH with Co(phen)3+3 by NADH dehydrogenase purified from Bacillus stearothermophilus

Tomokazu Matsue, Hiroshi Yamada, Hsien Chang Chang, Isamu Uchida

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Abstract

The NADH dehydrogenase purified from Bacillus stearothermophilus catalyzes effectively the electrochemical oxidation of NADH mediated by Co(phen)3+3. Stopped-flow measurements indicate that the rate constant for the oxidation of the reduced enzyme by Co(phen)3+3 is 5×106 M-1 s-1 and that for the oxidation of NADH by the oxidized enzyme > 3×107 M-1 s-1. The voltammograms, showing catalytic prepeaks, were simulated using the above rate constants.

Original languageEnglish
Pages (from-to)347-354
Number of pages8
JournalJournal of Electroanalytical Chemistry
Volume299
Issue number3
DOIs
Publication statusPublished - 1990 Dec

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry
  • Chemical Engineering(all)
  • Electrochemistry

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