The NADH dehydrogenase purified from Bacillus stearothermophilus catalyzes effectively the electrochemical oxidation of NADH mediated by Co(phen)3+3. Stopped-flow measurements indicate that the rate constant for the oxidation of the reduced enzyme by Co(phen)3+3 is 5×106 M-1 s-1 and that for the oxidation of NADH by the oxidized enzyme > 3×107 M-1 s-1. The voltammograms, showing catalytic prepeaks, were simulated using the above rate constants.
All Science Journal Classification (ASJC) codes
- Analytical Chemistry
- Chemical Engineering(all)