Electroporation microchips for gene transfection

Research output: Chapter in Book/Report/Conference proceedingConference contribution

2 Citations (Scopus)

Abstract

Electroporation (EP) microchips can increase gene transfection rate and cells survival rate. Electroporation is a kind of method in which DNA molecules can be delivered into cells by short electric pulses. Unlike the conventional electroporation device, the EP chips in this paper are loaded with a lower concentration of plasmids, and most cells will not be damaged or lysed by high electric field. Four different kinds of EP microchips were designed and fabricated by Micro Electro-Mechanical System (MEMS) technology. The first EP microchip was developed for delivering plasmids into suspension cells. The cells and LacZ plasmids flowed in a micro-channel and then electroporated by short electric pulses. Based on the cell morphology, the second microchip was focused on gene transfection to adherent cells. The third microchip was developed for reducing applied voltages and increasing the electric fields by interdigitated electrodes. The fourth one was designed for introducing the electrophoresis attracting force which increased the plasmid DNA concentration on the cell surfaces and further increased the transfection rate. The EP microchips could transfect plasmids into different cells, including primary cells and cell line.

Original languageEnglish
Title of host publicationEmerging Information Technology Conference 2005
Pages77-80
Number of pages4
DOIs
Publication statusPublished - 2005 Dec 1
EventEmerging Information Technology Conference 2005 - Taipei, Taiwan
Duration: 2005 Aug 152005 Aug 16

Publication series

NameEmerging Information Technology Conference 2005
Volume2005

Other

OtherEmerging Information Technology Conference 2005
CountryTaiwan
CityTaipei
Period05-08-1505-08-16

All Science Journal Classification (ASJC) codes

  • Engineering(all)

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