Elimination of undifferentiated human embryonic stem cells by cardiac glycosides

Yu Tsen Lin, Cheng Kai Wang, Shang Chih Yang, Shu Ching Hsu, Hsuan Lin, Fang Pei Chang, Tzu Chien Kuo, Chia Ning Shen, Po Ming Chiang, Michael Hsiao, Frank Leigh Lu, Jean Lu

Research output: Contribution to journalArticlepeer-review

8 Citations (Scopus)


An important safety concern in the use of human pluripotent stem cells (hPSCs) is tumorigenic risk, because these cells can form teratomas after an in vivo injection at ectopic sites. Several thousands of undifferentiated hPSCs are sufficient to induce teratomas in a mouse model. Thus, it is critical to remove all residue-undifferentiated hPSCs that have teratoma potential before the clinical application of hPSC-derived cells. In this study, our data demonstrated the cytotoxic effects of cardiac glycosides, such as digoxin, lanatoside C, bufalin, and proscillaridin A, in human embryonic stem cells (hESCs). This phenomenon was not observed in human bone marrow mesenchymal stem cells (hBMMSCs). Most importantly, digoxin and lanatoside C did not affect the stem cells' differentiation ability. Consistently, the viability of the hESC-derived MSCs, neurons, and endothelium cells was not affected by the digoxin and lanatoside C treatment. Furthermore, the in vivo experiments demonstrated that digoxin and lanatoside C prevented teratoma formation. To the best of our knowledge, this study is the first to describe the cytotoxicity and tumor prevention effects of cardiac glycosides in hESCs. Digoxin and lanatoside C are also the first FDA-approved drugs that demonstrated cytotoxicity in undifferentiated hESCs.

Original languageEnglish
Article number5289
JournalScientific reports
Issue number1
Publication statusPublished - 2017 Dec 1

All Science Journal Classification (ASJC) codes

  • General


Dive into the research topics of 'Elimination of undifferentiated human embryonic stem cells by cardiac glycosides'. Together they form a unique fingerprint.

Cite this