emm1/sequence type 28 strains of group A streptococci that express covR at early stationary phase are associated with increased growth and earlier speB secretion

Chuan Chiang-Ni, Po Xing Zheng, Yueh Ren Ho, Hsiu Mei Wu, Woei Jer Chuang, Yee Shin Lin, Ming T. Lin, Ching Chuan Liu, Jiunn Jong Wu

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Streptococcus pyogenes (group A streptococcus [GAS]) is a versatile human pathogen, and emm1/sequence type 28 (ST28) is the most frequently isolated type from GAS infections. The emm1/ST28 strain is associated with necrotizing fasciitis and streptococcal toxic shock syndrome. Growth-phase regulation is one of the important regulatory mechanisms in GAS, which controls gene expression at restricted phases of growth. CovRS, a two-component regulatory system, is considered the regulator of streptococcal pyrogenic exotoxin B (SpeB) and is thought to be activated in the exponential phase of growth. In the present study, Northern hybridization analysis showed that 52% of the analyzed GAS strains expressed covR at the exponential phase, but 48% of the strains expressed covR at the early stationary phase of growth. Strains transcribing covR at the early stationary phase showed better growth and earlier SpeB expression than the other group of strains. Multilocus sequence typing and pulsed-field gel electrophoresis analysis showed only emm1/ST28 strains (which comprise a clonal cluster) were expressing covR at the early stationary phase of growth, indicating that emm1/ST28 strains have special characteristics which may be related to their worldwide distribution.

Original languageEnglish
Pages (from-to)3161-3169
Number of pages9
JournalJournal of Clinical Microbiology
Volume47
Issue number10
DOIs
Publication statusPublished - 2009 Oct 1

Fingerprint

Streptococcus
Growth
Necrotizing Fasciitis
Multilocus Sequence Typing
Streptococcus pyogenes
Pulsed Field Gel Electrophoresis
Septic Shock
Gene Expression
Infection

All Science Journal Classification (ASJC) codes

  • Microbiology (medical)

Cite this

@article{11896c88df7f425b978140d27a1e0f5c,
title = "emm1/sequence type 28 strains of group A streptococci that express covR at early stationary phase are associated with increased growth and earlier speB secretion",
abstract = "Streptococcus pyogenes (group A streptococcus [GAS]) is a versatile human pathogen, and emm1/sequence type 28 (ST28) is the most frequently isolated type from GAS infections. The emm1/ST28 strain is associated with necrotizing fasciitis and streptococcal toxic shock syndrome. Growth-phase regulation is one of the important regulatory mechanisms in GAS, which controls gene expression at restricted phases of growth. CovRS, a two-component regulatory system, is considered the regulator of streptococcal pyrogenic exotoxin B (SpeB) and is thought to be activated in the exponential phase of growth. In the present study, Northern hybridization analysis showed that 52{\%} of the analyzed GAS strains expressed covR at the exponential phase, but 48{\%} of the strains expressed covR at the early stationary phase of growth. Strains transcribing covR at the early stationary phase showed better growth and earlier SpeB expression than the other group of strains. Multilocus sequence typing and pulsed-field gel electrophoresis analysis showed only emm1/ST28 strains (which comprise a clonal cluster) were expressing covR at the early stationary phase of growth, indicating that emm1/ST28 strains have special characteristics which may be related to their worldwide distribution.",
author = "Chuan Chiang-Ni and Zheng, {Po Xing} and Ho, {Yueh Ren} and Wu, {Hsiu Mei} and Chuang, {Woei Jer} and Lin, {Yee Shin} and Lin, {Ming T.} and Liu, {Ching Chuan} and Wu, {Jiunn Jong}",
year = "2009",
month = "10",
day = "1",
doi = "10.1128/JCM.00202-09",
language = "English",
volume = "47",
pages = "3161--3169",
journal = "Journal of Clinical Microbiology",
issn = "0095-1137",
publisher = "American Society for Microbiology",
number = "10",

}

emm1/sequence type 28 strains of group A streptococci that express covR at early stationary phase are associated with increased growth and earlier speB secretion. / Chiang-Ni, Chuan; Zheng, Po Xing; Ho, Yueh Ren; Wu, Hsiu Mei; Chuang, Woei Jer; Lin, Yee Shin; Lin, Ming T.; Liu, Ching Chuan; Wu, Jiunn Jong.

In: Journal of Clinical Microbiology, Vol. 47, No. 10, 01.10.2009, p. 3161-3169.

Research output: Contribution to journalArticle

TY - JOUR

T1 - emm1/sequence type 28 strains of group A streptococci that express covR at early stationary phase are associated with increased growth and earlier speB secretion

AU - Chiang-Ni, Chuan

AU - Zheng, Po Xing

AU - Ho, Yueh Ren

AU - Wu, Hsiu Mei

AU - Chuang, Woei Jer

AU - Lin, Yee Shin

AU - Lin, Ming T.

AU - Liu, Ching Chuan

AU - Wu, Jiunn Jong

PY - 2009/10/1

Y1 - 2009/10/1

N2 - Streptococcus pyogenes (group A streptococcus [GAS]) is a versatile human pathogen, and emm1/sequence type 28 (ST28) is the most frequently isolated type from GAS infections. The emm1/ST28 strain is associated with necrotizing fasciitis and streptococcal toxic shock syndrome. Growth-phase regulation is one of the important regulatory mechanisms in GAS, which controls gene expression at restricted phases of growth. CovRS, a two-component regulatory system, is considered the regulator of streptococcal pyrogenic exotoxin B (SpeB) and is thought to be activated in the exponential phase of growth. In the present study, Northern hybridization analysis showed that 52% of the analyzed GAS strains expressed covR at the exponential phase, but 48% of the strains expressed covR at the early stationary phase of growth. Strains transcribing covR at the early stationary phase showed better growth and earlier SpeB expression than the other group of strains. Multilocus sequence typing and pulsed-field gel electrophoresis analysis showed only emm1/ST28 strains (which comprise a clonal cluster) were expressing covR at the early stationary phase of growth, indicating that emm1/ST28 strains have special characteristics which may be related to their worldwide distribution.

AB - Streptococcus pyogenes (group A streptococcus [GAS]) is a versatile human pathogen, and emm1/sequence type 28 (ST28) is the most frequently isolated type from GAS infections. The emm1/ST28 strain is associated with necrotizing fasciitis and streptococcal toxic shock syndrome. Growth-phase regulation is one of the important regulatory mechanisms in GAS, which controls gene expression at restricted phases of growth. CovRS, a two-component regulatory system, is considered the regulator of streptococcal pyrogenic exotoxin B (SpeB) and is thought to be activated in the exponential phase of growth. In the present study, Northern hybridization analysis showed that 52% of the analyzed GAS strains expressed covR at the exponential phase, but 48% of the strains expressed covR at the early stationary phase of growth. Strains transcribing covR at the early stationary phase showed better growth and earlier SpeB expression than the other group of strains. Multilocus sequence typing and pulsed-field gel electrophoresis analysis showed only emm1/ST28 strains (which comprise a clonal cluster) were expressing covR at the early stationary phase of growth, indicating that emm1/ST28 strains have special characteristics which may be related to their worldwide distribution.

UR - http://www.scopus.com/inward/record.url?scp=70349651747&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=70349651747&partnerID=8YFLogxK

U2 - 10.1128/JCM.00202-09

DO - 10.1128/JCM.00202-09

M3 - Article

C2 - 19710271

AN - SCOPUS:70349651747

VL - 47

SP - 3161

EP - 3169

JO - Journal of Clinical Microbiology

JF - Journal of Clinical Microbiology

SN - 0095-1137

IS - 10

ER -