Endogenous latent membrane protein 1 in Epstein-Barr virus-infected nasopharyngeal carcinoma cells attracts T lymphocytes through upregulation of multiple chemokines

Hsiao Ching Lai, Jenn Ren Hsiao, Chaio Wei Chen, Shih Yi Wu, Chien Hsun Lee, Ih Jen Su, Kenzo Takada, Yao Chang

Research output: Contribution to journalArticlepeer-review

15 Citations (Scopus)

Abstract

Tumor-infiltrating T lymphocytes are considered to facilitate development of Epstein-Barr virus (EBV)-associated nasopharyngeal carcinoma (NPC), but how EBV in NPC tumor cells directs T cell infiltration remains unclear. Here we compare EBV-infected NPC cells with and without spontaneous expression of viral latent membrane protein 1 (LMP1) and find that culture supernatants of LMP1-positive NPC cells exert enhanced chemoattraction to primary T cells. Knockdown of endogenous LMP1 in the cells suppresses the chemotactic activity. Endogenous LMP1 in NPC cells upregulates multiple chemokines, among which MIP-1α, MIP-1β and IL-8 contribute to T cell chemotaxis. We further reveal that LMP1-induced production of MIP-1α and MIP-1β in NPC cells requires not only two carboxyl-terminal activation regions of LMP1 but also their downstream NF-κB and JNK pathways. This study corroborates that endogenous LMP1 in EBV-infected NPC cells induces multiple chemokines to promote T cell recruitment and perhaps other pathogenic events in NPC.

Original languageEnglish
Pages (from-to)464-473
Number of pages10
JournalVirology
Volume405
Issue number2
DOIs
Publication statusPublished - 2010 Sep 30

All Science Journal Classification (ASJC) codes

  • Virology

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