Engineered Nanostructures of Haptens Lead to Unexpected Formation of Membrane Nanotubes Connecting Rat Basophilic Leukemia Cells

Jie-Ren Li, Shailise S. Ross, Yang Liu, Ying X. Liu, Kang Hsin Wang, Huan Yuan Chen, Fu Tong Liu, Ted A. Laurence, Gang Yu Liu

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

A recent finding reports that co-stimulation of the high-affinity immunoglobulin E (IgE) receptor (Fc∈RI) and the chemokine receptor 1 (CCR1) triggered formation of membrane nanotubes among bone-marrow-derived mast cells. The co-stimulation was attained using corresponding ligands: IgE binding antigen and macrophage inflammatory protein 1α (MIP1 α), respectively. However, this approach failed to trigger formation of nanotubes among rat basophilic leukemia (RBL) cells due to the lack of CCR1 on the cell surface (Int. Immunol. 2010, 22 (2), 113-128). RBL cells are frequently used as a model for mast cells and are best known for antibody-mediated activation via Fc∈RI. This work reports the successful formation of membrane nanotubes among RBLs using only one stimulus, a hapten of 2,4-dinitrophenyl (DNP) molecules, which are presented as nanostructures with our designed spatial arrangements. This observation underlines the significance of the local presentation of ligands in the context of impacting the cellular signaling cascades. In the case of RBL, certain DNP nanostructures suppress antigen-induced degranulation and facilitate the rearrangement of the cytoskeleton to form nanotubes. These results demonstrate an important scientific concept; engineered nanostructures enable cellular signaling cascades, where current technologies encounter great difficulties. More importantly, nanotechnology offers a new platform to selectively activate and/or inhibit desired cellular signaling cascades.

Original languageEnglish
Pages (from-to)6738-6746
Number of pages9
JournalACS Nano
Volume9
Issue number7
DOIs
Publication statusPublished - 2015 Jul 28

Fingerprint

leukemias
Haptens
Cell signaling
Nanotubes
rats
Rats
Nanostructures
nanotubes
membranes
Membranes
cascades
Chemokine Receptors
antigens
Antigens
cells
stimulation
Ligands
Macrophage Inflammatory Proteins
IgE Receptors
ligands

All Science Journal Classification (ASJC) codes

  • Materials Science(all)
  • Engineering(all)
  • Physics and Astronomy(all)

Cite this

Li, Jie-Ren ; Ross, Shailise S. ; Liu, Yang ; Liu, Ying X. ; Wang, Kang Hsin ; Chen, Huan Yuan ; Liu, Fu Tong ; Laurence, Ted A. ; Liu, Gang Yu. / Engineered Nanostructures of Haptens Lead to Unexpected Formation of Membrane Nanotubes Connecting Rat Basophilic Leukemia Cells. In: ACS Nano. 2015 ; Vol. 9, No. 7. pp. 6738-6746.
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abstract = "A recent finding reports that co-stimulation of the high-affinity immunoglobulin E (IgE) receptor (Fc∈RI) and the chemokine receptor 1 (CCR1) triggered formation of membrane nanotubes among bone-marrow-derived mast cells. The co-stimulation was attained using corresponding ligands: IgE binding antigen and macrophage inflammatory protein 1α (MIP1 α), respectively. However, this approach failed to trigger formation of nanotubes among rat basophilic leukemia (RBL) cells due to the lack of CCR1 on the cell surface (Int. Immunol. 2010, 22 (2), 113-128). RBL cells are frequently used as a model for mast cells and are best known for antibody-mediated activation via Fc∈RI. This work reports the successful formation of membrane nanotubes among RBLs using only one stimulus, a hapten of 2,4-dinitrophenyl (DNP) molecules, which are presented as nanostructures with our designed spatial arrangements. This observation underlines the significance of the local presentation of ligands in the context of impacting the cellular signaling cascades. In the case of RBL, certain DNP nanostructures suppress antigen-induced degranulation and facilitate the rearrangement of the cytoskeleton to form nanotubes. These results demonstrate an important scientific concept; engineered nanostructures enable cellular signaling cascades, where current technologies encounter great difficulties. More importantly, nanotechnology offers a new platform to selectively activate and/or inhibit desired cellular signaling cascades.",
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Li, J-R, Ross, SS, Liu, Y, Liu, YX, Wang, KH, Chen, HY, Liu, FT, Laurence, TA & Liu, GY 2015, 'Engineered Nanostructures of Haptens Lead to Unexpected Formation of Membrane Nanotubes Connecting Rat Basophilic Leukemia Cells', ACS Nano, vol. 9, no. 7, pp. 6738-6746. https://doi.org/10.1021/acsnano.5b02270

Engineered Nanostructures of Haptens Lead to Unexpected Formation of Membrane Nanotubes Connecting Rat Basophilic Leukemia Cells. / Li, Jie-Ren; Ross, Shailise S.; Liu, Yang; Liu, Ying X.; Wang, Kang Hsin; Chen, Huan Yuan; Liu, Fu Tong; Laurence, Ted A.; Liu, Gang Yu.

In: ACS Nano, Vol. 9, No. 7, 28.07.2015, p. 6738-6746.

Research output: Contribution to journalArticle

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