TY - JOUR
T1 - Engineering cyanobacteria with enhanced growth in simulated flue gases for high-yield bioethanol production
AU - Chou, Hsiang Hui
AU - Su, Hsiang Yen
AU - Chow, Te Jin
AU - Lee, Tse Min
AU - Cheng, Wen Hsi
AU - Chang, Jo Shu
AU - Chen, Hsien Jung
N1 - Funding Information:
This work was supported by grants NSC103-2623-E-242 -001-ET, MOST 106-3113-E-006-011, MOST 107-2221-E-006 -112 -MY3, MOST 107-3113-E-006-009, and MOST 109-3116-F-006 -016 -CC1 from Taiwan's Ministry of Science and Technology.
Funding Information:
This work was supported by grants NSC103-2623-E-242 -001-ET , MOST 106-3113-E-006-011 , MOST 107-2221-E-006 -112 -MY3 , MOST 107-3113-E-006-009 , and MOST 109-3116-F-006 -016 -CC1 from Taiwan’s Ministry of Science and Technology .
Publisher Copyright:
© 2020 Elsevier B.V.
Copyright:
Copyright 2020 Elsevier B.V., All rights reserved.
PY - 2021/1/15
Y1 - 2021/1/15
N2 - Flue gases emitted from industrial plants mainly contribute to global CO2 emissions. Sequestrations of CO2 from the flue gas could reduce the impact of CO2 on global warming. In this study, recombinant cyanobacterial strains with enhanced photosynthetic activity, cell growth, and ethanol production were generated by co-overexpressing ictB, ecaA, and groESL, along with a heterologous ethanol synthesis pathway (pdc-adhII genes from Zymomonas mobilis) in S. elongatus PCC7942. The engineered S. elongatus PCC7942 exhibited a significant improvement in cell growth and ethanol production under a simulated flue gas consisted of 25% CO2, 80−90 ppm SO2, 90−100 ppm NO. The present work represents the first attempt of direct photoconversion of CO2 from flue gases to ethanol via expression of ictB, ecaA, groESL, and pdc-adhII in S. elongatus. The transgenic cyanobacterium becomes useful for sequestrations of CO2 directly from flue gases with the simultaneous production of bioethanol.
AB - Flue gases emitted from industrial plants mainly contribute to global CO2 emissions. Sequestrations of CO2 from the flue gas could reduce the impact of CO2 on global warming. In this study, recombinant cyanobacterial strains with enhanced photosynthetic activity, cell growth, and ethanol production were generated by co-overexpressing ictB, ecaA, and groESL, along with a heterologous ethanol synthesis pathway (pdc-adhII genes from Zymomonas mobilis) in S. elongatus PCC7942. The engineered S. elongatus PCC7942 exhibited a significant improvement in cell growth and ethanol production under a simulated flue gas consisted of 25% CO2, 80−90 ppm SO2, 90−100 ppm NO. The present work represents the first attempt of direct photoconversion of CO2 from flue gases to ethanol via expression of ictB, ecaA, groESL, and pdc-adhII in S. elongatus. The transgenic cyanobacterium becomes useful for sequestrations of CO2 directly from flue gases with the simultaneous production of bioethanol.
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U2 - 10.1016/j.bej.2020.107823
DO - 10.1016/j.bej.2020.107823
M3 - Article
AN - SCOPUS:85094598885
SN - 1369-703X
VL - 165
JO - Biochemical Engineering Journal
JF - Biochemical Engineering Journal
M1 - 107823
ER -