Engineering of dual-functional hybrid glucanases

Wei Chun Liu, Yu Shiun Lin, Wen Yih Jeng, Je Hsin Chen, Andrew H.J. Wang, Lie Fen Shyur

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

1,3-1,4-β-d-Glucanase (lichenase) and 1,3-β-d-glucanase (laminarinase) are fibrolytic enzymes which play an important role in the hydrolysis of polysaccharide components. Both of these glucanases have been employed in a number of industrial applications. This study aims to improve or combine the novel properties of both glucanases in an attempt to create desirable hybrid enzymes with economic benefits for industrial applications. A truncated and mutated 1,3-1,4-β-d-glucanase gene (TFsW203F) from Fibrobacter succinogenes, and a 1,3-β-d-glucanase gene (TmLam) from hyperthermophilic Thermotoga maritima were used as target enzymes. The substrate-binding domains (TmB1 and TmB2) and the catalytic domain (TmLamCD) of TmLam were ligated to the N- or C-terminus of TFsW203F to create four hybrid enzymes, TmB 1-TFsW203F, TFsW203F-TmB2, TmB 1-TFsW203F-TmB2 and TFsW203F- TmLamCD. The results obtained from kinetic studies show that increased specific activities and turnover rate for lichenan and laminarin were observed in TmB1-TFsW203F-TmB2 and TFs W203F-TmLamCD, respectively. Furthermore, fluorescence and circular dichroism spectrometric analyses indicated that the hybrid TFs W203F-TmLamCD was structurally more stable than the parental TFsW203F, which was attributed to an improved thermal tolerance of the hybrid enzyme. This study has been successful in creating bifunctional hybrid glucanases with dual substrate catalytic functions which warrant further evaluation of their possible use in industrial applications.

Original languageEnglish
Pages (from-to)771-780
Number of pages10
JournalProtein Engineering, Design and Selection
Volume25
Issue number11
DOIs
Publication statusPublished - 2012 Nov 1

Fingerprint

Enzymes
Industrial applications
Fibrobacter
Genes
Thermotoga maritima
Cellulases
Dichroism
Substrates
Polysaccharides
Circular Dichroism
Hydrolysis
Catalytic Domain
Fluorescence
Economics
Kinetics

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Bioengineering
  • Biochemistry
  • Molecular Biology

Cite this

Liu, W. C., Lin, Y. S., Jeng, W. Y., Chen, J. H., Wang, A. H. J., & Shyur, L. F. (2012). Engineering of dual-functional hybrid glucanases. Protein Engineering, Design and Selection, 25(11), 771-780. https://doi.org/10.1093/protein/gzs083
Liu, Wei Chun ; Lin, Yu Shiun ; Jeng, Wen Yih ; Chen, Je Hsin ; Wang, Andrew H.J. ; Shyur, Lie Fen. / Engineering of dual-functional hybrid glucanases. In: Protein Engineering, Design and Selection. 2012 ; Vol. 25, No. 11. pp. 771-780.
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abstract = "1,3-1,4-β-d-Glucanase (lichenase) and 1,3-β-d-glucanase (laminarinase) are fibrolytic enzymes which play an important role in the hydrolysis of polysaccharide components. Both of these glucanases have been employed in a number of industrial applications. This study aims to improve or combine the novel properties of both glucanases in an attempt to create desirable hybrid enzymes with economic benefits for industrial applications. A truncated and mutated 1,3-1,4-β-d-glucanase gene (TFsW203F) from Fibrobacter succinogenes, and a 1,3-β-d-glucanase gene (TmLam) from hyperthermophilic Thermotoga maritima were used as target enzymes. The substrate-binding domains (TmB1 and TmB2) and the catalytic domain (TmLamCD) of TmLam were ligated to the N- or C-terminus of TFsW203F to create four hybrid enzymes, TmB 1-TFsW203F, TFsW203F-TmB2, TmB 1-TFsW203F-TmB2 and TFsW203F- TmLamCD. The results obtained from kinetic studies show that increased specific activities and turnover rate for lichenan and laminarin were observed in TmB1-TFsW203F-TmB2 and TFs W203F-TmLamCD, respectively. Furthermore, fluorescence and circular dichroism spectrometric analyses indicated that the hybrid TFs W203F-TmLamCD was structurally more stable than the parental TFsW203F, which was attributed to an improved thermal tolerance of the hybrid enzyme. This study has been successful in creating bifunctional hybrid glucanases with dual substrate catalytic functions which warrant further evaluation of their possible use in industrial applications.",
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Liu, WC, Lin, YS, Jeng, WY, Chen, JH, Wang, AHJ & Shyur, LF 2012, 'Engineering of dual-functional hybrid glucanases', Protein Engineering, Design and Selection, vol. 25, no. 11, pp. 771-780. https://doi.org/10.1093/protein/gzs083

Engineering of dual-functional hybrid glucanases. / Liu, Wei Chun; Lin, Yu Shiun; Jeng, Wen Yih; Chen, Je Hsin; Wang, Andrew H.J.; Shyur, Lie Fen.

In: Protein Engineering, Design and Selection, Vol. 25, No. 11, 01.11.2012, p. 771-780.

Research output: Contribution to journalArticle

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AU - Liu, Wei Chun

AU - Lin, Yu Shiun

AU - Jeng, Wen Yih

AU - Chen, Je Hsin

AU - Wang, Andrew H.J.

AU - Shyur, Lie Fen

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