Engineering pyridoxal kinase PdxY-integrated Escherichia coli strain and optimization for high-level 5-aminolevulinic acid production

Chengfeng Xue, Tzu Hsuan Yu, I. Son Ng

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1 Citation (Scopus)

Abstract

Recently, 5-aminolevulinic acid (ALA) has gained significant attention in research due to its extensive applications in agriculture, aquaculture, cosmetics, and photodynamic therapy for cancer. Microbial production of ALA is a green, high-efficient, and sustainable process. Herein, we have engineered a new strain of Escherichia coli called PdxY Integrative E. coli Enhancer (PIECE). In this strain, the super salvage pathway was fine-tuned to enhance the regeneration of pyridoxal 5′-phosphate (PLP), on which the activity of ALA synthase (ALAS) is highly dependent. The PIECE strain, harboring ALA synthase (ALAS) from Rhodobacter capsulatus, produced 1.99 g/L ALA and 5.15 g/L biomass, that was a 4.33-fold increase in ALA production and a 1.84-fold increase in biomass compared to the parent strain. The metabolic flux analysis indicated that PIECE would redirect more carbon into the tricarboxylic acid (TCA) cycle and generate more glycine and glutamate, thereby increasing the ALA and biomass. Finally, the maximum ALA yield of 8.21 g/L and a productivity of 0.228 g/L/h were obtained by using fed-batch fermentation. The results showed that PIECE was not only feasible for high-level ALA production but also had significant potential to reprogram the carbon flux in the TCA cycle to produce value-added chemicals in the future.

Original languageEnglish
Pages (from-to)49-58
Number of pages10
JournalJournal of the Taiwan Institute of Chemical Engineers
Volume120
DOIs
Publication statusPublished - 2021 Mar

All Science Journal Classification (ASJC) codes

  • Chemistry(all)
  • Chemical Engineering(all)

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