TY - JOUR
T1 - Enhanced carbon capture and utilization (CCU) using heterologous carbonic anhydrase in Chlamydomonas reinhardtii for lutein and lipid production
AU - Lin, Jia Yi
AU - Sri Wahyu Effendi, Sefli
AU - Ng, I. Son
N1 - Funding Information:
The authors are grateful for the financial support received from the Ministry of Science and Technology (MOST 110-2221-E-006-030-MY3, MOST 108-2221-E-006-004-MY3 and MOST 108-2621-M-006-015) in Taiwan. The authors also thank for the support of Elemental Analyzer equipment belonging to the Core Facility Center of National Cheng Kung University under granting from MOST 110-2731-M-006-001.
Publisher Copyright:
© 2022 Elsevier Ltd
PY - 2022/5
Y1 - 2022/5
N2 - Chlamydomonas reinhardtii is a model microalga that has a higher growth rate and produces high levels of lutein and lipids, but biomass production is limited. Carbonic anhydrase (CA) converts atmospheric CO2 to bicarbonate which is crucial for carbon‐concentrating mechanism (CCM) in microalgae and boosts cell density. Therefore, C. reinhardtii harboring the heterologous CA from Mesorhizobium loti (MlCA) and Sulfurihydrogenibium yellowstonense (SyCA) were explored to increase CO2 capture and utilization (CCU) through different culture devices. Genetically modified C. reinhardtii was able to grow from mixotrophic to autotrophic conditions. Subsequently, biomass, lutein, and lipid were maximized to OD680 of 4.56, 21.32 mg/L and 672 mg/L using photo-bioreactor (PBR) with 5% CO2. Moreover, CO2 assimilation rate was 2.748 g-CO2/g-DCW and 2.792 g-CO2/g-DCW under mixotrophic and autotrophic conditions, respectively. The biomass accumulation correlated with CA activity. In addition, the transcript levels of major genes in metabolic pathways of lutein and lipid were dramatically increased.
AB - Chlamydomonas reinhardtii is a model microalga that has a higher growth rate and produces high levels of lutein and lipids, but biomass production is limited. Carbonic anhydrase (CA) converts atmospheric CO2 to bicarbonate which is crucial for carbon‐concentrating mechanism (CCM) in microalgae and boosts cell density. Therefore, C. reinhardtii harboring the heterologous CA from Mesorhizobium loti (MlCA) and Sulfurihydrogenibium yellowstonense (SyCA) were explored to increase CO2 capture and utilization (CCU) through different culture devices. Genetically modified C. reinhardtii was able to grow from mixotrophic to autotrophic conditions. Subsequently, biomass, lutein, and lipid were maximized to OD680 of 4.56, 21.32 mg/L and 672 mg/L using photo-bioreactor (PBR) with 5% CO2. Moreover, CO2 assimilation rate was 2.748 g-CO2/g-DCW and 2.792 g-CO2/g-DCW under mixotrophic and autotrophic conditions, respectively. The biomass accumulation correlated with CA activity. In addition, the transcript levels of major genes in metabolic pathways of lutein and lipid were dramatically increased.
UR - https://www.scopus.com/pages/publications/85126600952
UR - https://www.scopus.com/pages/publications/85126600952#tab=citedBy
U2 - 10.1016/j.biortech.2022.127009
DO - 10.1016/j.biortech.2022.127009
M3 - Article
C2 - 35304253
AN - SCOPUS:85126600952
SN - 0960-8524
VL - 351
JO - Bioresource technology
JF - Bioresource technology
M1 - 127009
ER -