TY - JOUR
T1 - Enhanced carbon capture, lipid and lutein production in Chlamydomonas reinhardtii under meso-thermophilic conditions using chaperone and CRISPRi system
AU - Lin, Jia Yi
AU - Ng, I. Son
N1 - Funding Information:
The authors are grateful for the financial support received from the Ministry of Science and Technology (MOST 110-2221-E-006-030-MY3 and NSTC 111-2221-E-006-012-MY3) in Taiwan. The authors also thank for the support of Elemental Analyzer equipment EA000600 belonging to the Core Facility Center of National Cheng Kung University under granting from NSTC 112-2740-M-006-001.
Funding Information:
The authors are grateful for the financial support received from the Ministry of Science and Technology (MOST 110-2221-E-006-030-MY3 and NSTC 111-2221-E-006-012-MY3) in Taiwan. The authors also thank for the support of Elemental Analyzer equipment EA000600 belonging to the Core Facility Center of National Cheng Kung University under granting from NSTC 112-2740-M-006-001.
Publisher Copyright:
© 2023 Elsevier Ltd
PY - 2023/9
Y1 - 2023/9
N2 - Microalgae are widely recognized as a promising bioresource for producing renewable fuels and chemicals. Microalgal biorefinery has tremendous potential for incorporation into circular bioeconomy, including sustainability, cascading use, and waste reduction. In this study, genetic engineering was used to enhance the growth, lipid and lutein productivity of Chlamydomonas reinhardtii including strains of CC400, PY9, pCHS, and PG. Notably, CRISPRi mediated on phosphoenolpyruvate carboxylase (PEPC1) gene to down-regulate the branch pathway from glycolysis to partitioning more carbon flux to lipid was explored under meso-thermophilic condition. The best chassis PGi, which has overexpressed chaperone GroELS and applied CRISPRi resulting in the highest biomass of 2.56 g/L and also boosted the lipids and lutein with 893 and 23.5 mg/L, respectively at 35°C. Finally, all strains with CRISPRi exhibited higher transcriptional levels of the crucial genes from photosynthesis, starch, lipid and lutein metabolism, thus reaching a CO2 assimilation of 1.087 g-CO2/g-DCW in mixotrophic condition.
AB - Microalgae are widely recognized as a promising bioresource for producing renewable fuels and chemicals. Microalgal biorefinery has tremendous potential for incorporation into circular bioeconomy, including sustainability, cascading use, and waste reduction. In this study, genetic engineering was used to enhance the growth, lipid and lutein productivity of Chlamydomonas reinhardtii including strains of CC400, PY9, pCHS, and PG. Notably, CRISPRi mediated on phosphoenolpyruvate carboxylase (PEPC1) gene to down-regulate the branch pathway from glycolysis to partitioning more carbon flux to lipid was explored under meso-thermophilic condition. The best chassis PGi, which has overexpressed chaperone GroELS and applied CRISPRi resulting in the highest biomass of 2.56 g/L and also boosted the lipids and lutein with 893 and 23.5 mg/L, respectively at 35°C. Finally, all strains with CRISPRi exhibited higher transcriptional levels of the crucial genes from photosynthesis, starch, lipid and lutein metabolism, thus reaching a CO2 assimilation of 1.087 g-CO2/g-DCW in mixotrophic condition.
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U2 - 10.1016/j.biortech.2023.129340
DO - 10.1016/j.biortech.2023.129340
M3 - Article
C2 - 37343802
AN - SCOPUS:85163145295
SN - 0960-8524
VL - 384
JO - Bioresource technology
JF - Bioresource technology
M1 - 129340
ER -
