Enhanced cell proliferation on biomedical titanium surfaces by laser ablation-induced micro- And nanoscale hybrid structures

Hwa-Teng Lee, Ching Chi Lin

Research output: Contribution to journalArticle

Abstract

The cell proliferation performance of pure titanium substrates was enhanced by modifying the surface morphology using an ultraviolet laser with a wavelength of 355 nm and travel speeds ranging from 103300 mm/sec. Rat calvarial osteoblast cells were cultured on the sample surfaces for 137 days. The cell proliferation was investigated via 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assays. Scanning electron microscopy observations showed that the laser ablation (LA) surfaces had a hybrid micro- and nanoscale structure consisting of microscale grooves with nanoscale agglomerations on their surface. For a low laser travel speed of 10 mm/sec, the grooves had a width of approximately 5.44310.03 µm. For the maximum travel speed of 300 mm/sec, the grooves reduced in height, but increased in width to around 10.97320.06 µm. The agglomerations on the grooves had a size of around 303100 nm; with larger agglomerations being formed at a lower laser travel speed. The XRD analysis results revealed the presence of titanium compounds (TiO and TiN0.3) on the LA surfaces ablated at lower travel speeds of 10 mm/sec and 50 mm/sec, respectively. The MTT measurements showed that the LA samples yielded a better cell proliferation rate than a sandblasted acid-etched titanium sample or a machined titanium sample. Furthermore, the cell proliferation rate increased with a decreasing laser travel speed. In general, the present results confirm the feasibility of laser ablation surface modification as a means of promoting the cell proliferation rate on titanium bioimplants.

Original languageEnglish
Pages (from-to)1799-1806
Number of pages8
JournalMaterials Transactions
Volume60
Issue number9
DOIs
Publication statusPublished - 2019 Jan 1

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hybrid structures
Cell proliferation
Laser ablation
Titanium
microbalances
travel
laser ablation
titanium
grooves
agglomeration
Agglomeration
Lasers
Titanium compounds
titanium compounds
lasers
Ultraviolet lasers
osteoblasts
Osteoblasts
ultraviolet lasers
cultured cells

All Science Journal Classification (ASJC) codes

  • Materials Science(all)
  • Condensed Matter Physics
  • Mechanics of Materials
  • Mechanical Engineering

Cite this

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abstract = "The cell proliferation performance of pure titanium substrates was enhanced by modifying the surface morphology using an ultraviolet laser with a wavelength of 355 nm and travel speeds ranging from 103300 mm/sec. Rat calvarial osteoblast cells were cultured on the sample surfaces for 137 days. The cell proliferation was investigated via 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assays. Scanning electron microscopy observations showed that the laser ablation (LA) surfaces had a hybrid micro- and nanoscale structure consisting of microscale grooves with nanoscale agglomerations on their surface. For a low laser travel speed of 10 mm/sec, the grooves had a width of approximately 5.44310.03 µm. For the maximum travel speed of 300 mm/sec, the grooves reduced in height, but increased in width to around 10.97320.06 µm. The agglomerations on the grooves had a size of around 303100 nm; with larger agglomerations being formed at a lower laser travel speed. The XRD analysis results revealed the presence of titanium compounds (TiO and TiN0.3) on the LA surfaces ablated at lower travel speeds of 10 mm/sec and 50 mm/sec, respectively. The MTT measurements showed that the LA samples yielded a better cell proliferation rate than a sandblasted acid-etched titanium sample or a machined titanium sample. Furthermore, the cell proliferation rate increased with a decreasing laser travel speed. In general, the present results confirm the feasibility of laser ablation surface modification as a means of promoting the cell proliferation rate on titanium bioimplants.",
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Enhanced cell proliferation on biomedical titanium surfaces by laser ablation-induced micro- And nanoscale hybrid structures. / Lee, Hwa-Teng; Lin, Ching Chi.

In: Materials Transactions, Vol. 60, No. 9, 01.01.2019, p. 1799-1806.

Research output: Contribution to journalArticle

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