Enhanced mobile hybridization of gold nanoparticles decorated with oligonucleotide in microchannel devices

Miao Hsing Hsu, Wei Feng Fang, Yu Hsuan Lai, Jing Tang Yang, Tsung Lin Tsai, Dar Bin Shieh

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Mobile hybridization is a concept proposed and verified herein. We have designed a microfluidic device that is capable of enhancing passive mixing through the morphology of micro-structures, positioned along the channels of the device. We investigated the capability of these structures to promote mobile hybridization of fluorophore-labeled target oligonucleotides to oligonucleotide gold-nanoparticle (Au-NP) probes. This process is monitored with fluorescence through the quenching of the fluorescent signal within the device as the target oligonucleotides become bound to the Au-NP probes. We evaluated the fluorescent intensity of a sample image that showed enhanced probability of mobile hybridization of the samples, which was completed in about 7.2 s. Mobile hybridization is thus much more effective than traditional static hybridization (reaction overnight) limited by molecular diffusion. This approach promises an improved hybridization of samples with these probes, and is beneficial for microfluidic-based systems for biomedical detection.

Original languageEnglish
Pages (from-to)2583-2587
Number of pages5
JournalLab on a Chip
Volume10
Issue number19
DOIs
Publication statusPublished - 2010 Oct 7

All Science Journal Classification (ASJC) codes

  • Bioengineering
  • Biochemistry
  • Chemistry(all)
  • Biomedical Engineering

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