Enhanced mobile hybridization of gold nanoparticles decorated with oligonucleotide in microchannel devices

Miao Hsing Hsu, Wei Feng Fang, Yu Hsuan Lai, Jing Tang Yang, Tsung Lin Tsai, Dar Bin Shieh

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

We have designed a microfluidic device that is capable of enhancing passive mixing through the micro-structures, positioned along the channels of the device. We investigated the capability of these structures to promote mobile hybridization of fluorophore-labeled target oligonucleotides to Au-NP probes. This process is monitored with fluorescence through the quenching of the fluorescent signal within the device as the target oligonucleotides become bound to the Au-NP probes. We evaluated the fluorescent intensity of a sample image which was completed in about 7.2 s. Mobile hybridization is thus much more effective than traditional static hybridization limited by molecular diffusion.

Original languageEnglish
Title of host publication14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010
Pages336-338
Number of pages3
Publication statusPublished - 2010 Dec 1
Event14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010 - Groningen, Netherlands
Duration: 2010 Oct 32010 Oct 7

Publication series

Name14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010
Volume1

Other

Other14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010
CountryNetherlands
CityGroningen
Period10-10-0310-10-07

All Science Journal Classification (ASJC) codes

  • Control and Systems Engineering

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