Enhanced myometrial autophagy in postpartum uterine involution

Keng-Fu Hsu, Hsien An Pan, Yu-Yun Hsu, Ching Ming Wu, Wen Ju Chung, Soon Cen Huang

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Objective: To understand the mechanisms of postpartum uterine involution, we investigated the uterine myometrial changes during pregnancy and the postpartum period. Materials and methods: Nine groups of uterine myometrial samples from mice (n=4) were collected on gestational Day 0 (nonpregnant), Day 1, Day 2, Day 7, Day 14, and Day 21 and on postpartum Day 1, Day 2, and Day 7. Human samples of uterine myometrium on term (n=1) and postpartum Day 1 (n=2) were also collected. Ki-67 immunostaining was used to determine myometrial proliferation. For cell hypertrophy analysis, organelle proteins, β-actin, prohibin, calnexin, and golgin-97 were analyzed by Western blotting. Terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling (TUNEL) and evaluation of activated caspase-3 expression by Western blot analysis assay were used to detect apoptosis. Autophagy was assayed via the evaluation of LC3 expression by Western blotting, immunohistochemistry, and autophagosomes by electron microscopy. Results: Uterine myocytes proliferated during the early stage of gestation with a peak at Day 2, whereas myocyte hypertrophy with increased cellular organelle production occurred gradually in later stages of pregnancy. Postpartum autophagy developed abruptly in uterine myocytes without obvious apoptosis. Conclusion: Autophagy of myocytes may play an important role in uterine involution. These results have implications for our understanding of myometrial functional adaptations during pregnancy and the physiological role of autophagy in the uterine remodeling events in the postpartum period.

Original languageEnglish
Pages (from-to)293-302
Number of pages10
JournalTaiwanese Journal of Obstetrics and Gynecology
Volume53
Issue number3
DOIs
Publication statusPublished - 2014 Sep 1

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Autophagy
Postpartum Period
Muscle Cells
Pregnancy
Western Blotting
Organelles
Hypertrophy
Calnexin
Apoptosis
DNA Nucleotidylexotransferase
Myometrium
Caspase 3
Actins
Electron Microscopy
Immunohistochemistry
Proteins

All Science Journal Classification (ASJC) codes

  • Obstetrics and Gynaecology

Cite this

Hsu, Keng-Fu ; Pan, Hsien An ; Hsu, Yu-Yun ; Wu, Ching Ming ; Chung, Wen Ju ; Huang, Soon Cen. / Enhanced myometrial autophagy in postpartum uterine involution. In: Taiwanese Journal of Obstetrics and Gynecology. 2014 ; Vol. 53, No. 3. pp. 293-302.
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Enhanced myometrial autophagy in postpartum uterine involution. / Hsu, Keng-Fu; Pan, Hsien An; Hsu, Yu-Yun; Wu, Ching Ming; Chung, Wen Ju; Huang, Soon Cen.

In: Taiwanese Journal of Obstetrics and Gynecology, Vol. 53, No. 3, 01.09.2014, p. 293-302.

Research output: Contribution to journalArticle

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