TY - JOUR
T1 - Enhanced production of microalgal lipids using a heterotrophic marine microalga Thraustochytrium sp. BM2
AU - Chen, Chun Yen
AU - Lee, Meng Hsiu
AU - Dong, Cheng Di
AU - Leong, Yoong Kit
AU - Chang, Jo Shu
N1 - Funding Information:
The authors gratefully acknowledge financial support received from Taiwan’s Ministry of Science and Technology (MOST) under grant numbers 108-3116-F-006-007-CC1 , 107-2221-E-006-112-MY3 , and 107-2621-M-006-003 .
Publisher Copyright:
© 2019
PY - 2020/2/15
Y1 - 2020/2/15
N2 - In this study, the potential of Thraustochytrium sp. BM2 for cost-effective lipid production was investigated. Lipid content and productivity of BM2 reached 76% and 37 mg/L/h when corn steep liquor (12.5 g/L) and glycerol (10 g/L) was used as nitrogen and carbon source respectively. Supplementing an optimal amount of sea salt (2% w/v) further enhanced the lipid content to 79%. The inoculum size and age were also evaluated for their effect on lipid accumulation. A 48-h pre-culture at 0.15 g/L loading further improved the lipid productivity to 43.86 mg/L/h (or 1.052 g/L/d), which is a 340% increase when compared with the control test. Thus, Thraustochytrium sp. BM2 strain could serve as a low-cost and high-lipid-yield heterotrophic lipid producer using glycerol (a by-product of biodiesel manufacturing process) as carbon source and an inexpensive nitrogen source (i.e., corn steep liquor).
AB - In this study, the potential of Thraustochytrium sp. BM2 for cost-effective lipid production was investigated. Lipid content and productivity of BM2 reached 76% and 37 mg/L/h when corn steep liquor (12.5 g/L) and glycerol (10 g/L) was used as nitrogen and carbon source respectively. Supplementing an optimal amount of sea salt (2% w/v) further enhanced the lipid content to 79%. The inoculum size and age were also evaluated for their effect on lipid accumulation. A 48-h pre-culture at 0.15 g/L loading further improved the lipid productivity to 43.86 mg/L/h (or 1.052 g/L/d), which is a 340% increase when compared with the control test. Thus, Thraustochytrium sp. BM2 strain could serve as a low-cost and high-lipid-yield heterotrophic lipid producer using glycerol (a by-product of biodiesel manufacturing process) as carbon source and an inexpensive nitrogen source (i.e., corn steep liquor).
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U2 - 10.1016/j.bej.2019.107429
DO - 10.1016/j.bej.2019.107429
M3 - Article
AN - SCOPUS:85074703443
VL - 154
JO - Biochemical Engineering Journal
JF - Biochemical Engineering Journal
SN - 1369-703X
M1 - 107429
ER -