Enhanced production of microalgal lipids using a heterotrophic marine microalga Thraustochytrium sp. BM2

Chun Yen Chen, Meng Hsiu Lee, Cheng Di Dong, Yoong Kit Leong, Jo Shu Chang

Research output: Contribution to journalArticle

Abstract

In this study, the potential of Thraustochytrium sp. BM2 for cost-effective lipid production was investigated. Lipid content and productivity of BM2 reached 76% and 37 mg/L/h when corn steep liquor (12.5 g/L) and glycerol (10 g/L) was used as nitrogen and carbon source respectively. Supplementing an optimal amount of sea salt (2% w/v) further enhanced the lipid content to 79%. The inoculum size and age were also evaluated for their effect on lipid accumulation. A 48-h pre-culture at 0.15 g/L loading further improved the lipid productivity to 43.86 mg/L/h (or 1.052 g/L/d), which is a 340% increase when compared with the control test. Thus, Thraustochytrium sp. BM2 strain could serve as a low-cost and high-lipid-yield heterotrophic lipid producer using glycerol (a by-product of biodiesel manufacturing process) as carbon source and an inexpensive nitrogen source (i.e., corn steep liquor).

Original languageEnglish
Article number107429
JournalBiochemical Engineering Journal
Volume154
DOIs
Publication statusPublished - 2020 Feb 15

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Lipids
Glycerol
Zea mays
Nitrogen
Carbon
Productivity
Costs and Cost Analysis
Biofuels
Biodiesel
Oceans and Seas
Byproducts
Costs
Salts

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Environmental Engineering
  • Bioengineering
  • Biomedical Engineering

Cite this

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title = "Enhanced production of microalgal lipids using a heterotrophic marine microalga Thraustochytrium sp. BM2",
abstract = "In this study, the potential of Thraustochytrium sp. BM2 for cost-effective lipid production was investigated. Lipid content and productivity of BM2 reached 76{\%} and 37 mg/L/h when corn steep liquor (12.5 g/L) and glycerol (10 g/L) was used as nitrogen and carbon source respectively. Supplementing an optimal amount of sea salt (2{\%} w/v) further enhanced the lipid content to 79{\%}. The inoculum size and age were also evaluated for their effect on lipid accumulation. A 48-h pre-culture at 0.15 g/L loading further improved the lipid productivity to 43.86 mg/L/h (or 1.052 g/L/d), which is a 340{\%} increase when compared with the control test. Thus, Thraustochytrium sp. BM2 strain could serve as a low-cost and high-lipid-yield heterotrophic lipid producer using glycerol (a by-product of biodiesel manufacturing process) as carbon source and an inexpensive nitrogen source (i.e., corn steep liquor).",
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Enhanced production of microalgal lipids using a heterotrophic marine microalga Thraustochytrium sp. BM2. / Chen, Chun Yen; Lee, Meng Hsiu; Dong, Cheng Di; Leong, Yoong Kit; Chang, Jo Shu.

In: Biochemical Engineering Journal, Vol. 154, 107429, 15.02.2020.

Research output: Contribution to journalArticle

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AU - Chang, Jo Shu

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