TY - JOUR
T1 - Enhanced sonodynamic therapy by carbon dots-shelled microbubbles with focused ultrasound
AU - Fan, Ching Hsiang
AU - Wu, Nan
AU - Yeh, Chih Kuang
N1 - Funding Information:
This work was supported by the National Science and Technology Council (NSTC) of Taiwan, under grants nos. 108-2221-E-007-040-MY3, 108-2221-E-007-041-MY3, 110-2221-E-007-019-MY3, 111-2321-B-002-014, 111-2221-E-007-019-MY3, 108-2638-M-002-001-MY2, and 111-2636-E-006-025. The authors also gratefully acknowledge the supports of Professors Y.-F. Huang, C.-C. Huang, H.-T. Chang for their help with experiments.
Publisher Copyright:
© 2023 The Author(s)
PY - 2023/3
Y1 - 2023/3
N2 - Sonodynamic therapy involving the non-invasive and local generation of lethal reactive oxygen species (ROS) via ultrasound (US) with sonosensitizers has been proposed as an emerging tumor therapy strategy. However, such therapy is usually associated with inertial cavitation and unnecessary damage to healthy tissue because current sonosensitizers have insufficient sensitivity to US. Here, we report the use of a new proposed sonosensitizer, carbon dots (C-dots), to assemble microbubbles with a gas core (C-dots MBs). As the C-dots were directly integrated into the MB shell, they could effectively absorb the energy of inertial cavitation and transfer it to ROS. Our results revealed the appearance of 1O2, •OH, and H2O2 after US irradiation of C-dots MBs. In in vitro experiments, treatment with C-dots MBs plus US induced lipid peroxidation, elevation of intracellular ROS, and apoptosis in 32.5%, 45.3%, and 50.1% of cells respectively. In an animal solid tumor model, treatment with C-dots MBs plus US resulted in a 3-fold and 2.5-fold increase in the proportion of ROS-damaged cells and apoptotic cells, respectively, compared to C-dots MBs alone. These results will pave the way for the design of novel multifunctional sonosensitizers for SDT tumor therapy.
AB - Sonodynamic therapy involving the non-invasive and local generation of lethal reactive oxygen species (ROS) via ultrasound (US) with sonosensitizers has been proposed as an emerging tumor therapy strategy. However, such therapy is usually associated with inertial cavitation and unnecessary damage to healthy tissue because current sonosensitizers have insufficient sensitivity to US. Here, we report the use of a new proposed sonosensitizer, carbon dots (C-dots), to assemble microbubbles with a gas core (C-dots MBs). As the C-dots were directly integrated into the MB shell, they could effectively absorb the energy of inertial cavitation and transfer it to ROS. Our results revealed the appearance of 1O2, •OH, and H2O2 after US irradiation of C-dots MBs. In in vitro experiments, treatment with C-dots MBs plus US induced lipid peroxidation, elevation of intracellular ROS, and apoptosis in 32.5%, 45.3%, and 50.1% of cells respectively. In an animal solid tumor model, treatment with C-dots MBs plus US resulted in a 3-fold and 2.5-fold increase in the proportion of ROS-damaged cells and apoptotic cells, respectively, compared to C-dots MBs alone. These results will pave the way for the design of novel multifunctional sonosensitizers for SDT tumor therapy.
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U2 - 10.1016/j.ultsonch.2023.106342
DO - 10.1016/j.ultsonch.2023.106342
M3 - Article
C2 - 36842213
AN - SCOPUS:85149059221
SN - 1350-4177
VL - 94
JO - Ultrasonics Sonochemistry
JF - Ultrasonics Sonochemistry
M1 - 106342
ER -