Enhancement of an electroporation system for gene delivery using electrophoresis with a planar electrode

Keng Shiang Huang, Yu-Cheng Lin, Chi Chang Su, Chun Sheng Fang

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

In this paper a new electroporation (EP) system is developed, which includes an EP microchip and a logic circuit, which combined with electrophoresis (ES), can provide site-specific enhancement of gene concentration. In this ES-EP microchip, an arc planar electrode provides the ES function for DNA attraction, and interdigitated array electrodes provide appropriate electric fields for the EP on the chip surface. In addition, the adherent cells can be manipulated in situ without detachment of the ES-EP microchip, which performs the "Lab on a chip". Experimental results have shown that the efficiency of gene transfection with an attracting-electric field (35.89%) becomes much higher than that without an attracting-electric field (16.62%). Cell numbers as low as 104 cells, and DNA as little as 4 μg are sufficient for evaluating the phenotypic effects following the over-expression of the introduced genes on the ES-EP microchip. The proposed system has the advantages of portability, cost-effectiveness, a high transfection rate and ease of operation.

Original languageEnglish
Pages (from-to)86-92
Number of pages7
JournalLab on a Chip
Volume7
Issue number1
DOIs
Publication statusPublished - 2007 Jan 1

Fingerprint

Gene Transfer Techniques
Electroporation
Electrophoresis
Electrodes
Microchip Electrophoresis
Genes
Electric fields
DNA
Transfection
Lab-on-a-chip
Logic circuits
Cost effectiveness
Cost-Benefit Analysis
Cell Count
Gene Expression

All Science Journal Classification (ASJC) codes

  • Bioengineering
  • Biochemistry
  • Chemistry(all)
  • Biomedical Engineering

Cite this

Huang, Keng Shiang ; Lin, Yu-Cheng ; Su, Chi Chang ; Fang, Chun Sheng. / Enhancement of an electroporation system for gene delivery using electrophoresis with a planar electrode. In: Lab on a Chip. 2007 ; Vol. 7, No. 1. pp. 86-92.
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Enhancement of an electroporation system for gene delivery using electrophoresis with a planar electrode. / Huang, Keng Shiang; Lin, Yu-Cheng; Su, Chi Chang; Fang, Chun Sheng.

In: Lab on a Chip, Vol. 7, No. 1, 01.01.2007, p. 86-92.

Research output: Contribution to journalArticle

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