TY - GEN
T1 - Enhancement of an electroporation system for gene delivery using electrophoresis with planar electrodes
AU - Huang, Keng Shiang
AU - Li, Min
AU - Su, Chi Chang
AU - Huang, Hau Hsuan
AU - Fang, Chun Sheng
AU - Lin, Yu Cheng
PY - 2005
Y1 - 2005
N2 - We developed a new electroporation system, including a microchip and a logic circuit, and combining with the function of electrophoresis, which can site-specific enhancement of the gene concentration. We have demonstrated that the electroporation microchip could enhance and target in vitro gene transfection for cell lines. In this micro-device, the outer electrodes could provide the electrophoresis function for DNA attraction, and the inner electrodes could provide appropriate electric fields for the electroporation on the chip surface. The electrostatic force can be designed into specific regions, where the DNA plasmids are attracted to provide the region-targeting function. This study successfully demonstrates that the electrostatic force can attract DNA plasmids to the cell surface and highly enhance the gene delivery. Experimental results showed that the efficiency of gene transfection with an attracting-electric field become much higher than that without an attracting-electric field. Furthermore, the adherent cells could be manipulated in situ without detachment by this EP microchip. The system has several advantages of portable, cost-effective, high transfection rate and easy operation.
AB - We developed a new electroporation system, including a microchip and a logic circuit, and combining with the function of electrophoresis, which can site-specific enhancement of the gene concentration. We have demonstrated that the electroporation microchip could enhance and target in vitro gene transfection for cell lines. In this micro-device, the outer electrodes could provide the electrophoresis function for DNA attraction, and the inner electrodes could provide appropriate electric fields for the electroporation on the chip surface. The electrostatic force can be designed into specific regions, where the DNA plasmids are attracted to provide the region-targeting function. This study successfully demonstrates that the electrostatic force can attract DNA plasmids to the cell surface and highly enhance the gene delivery. Experimental results showed that the efficiency of gene transfection with an attracting-electric field become much higher than that without an attracting-electric field. Furthermore, the adherent cells could be manipulated in situ without detachment by this EP microchip. The system has several advantages of portable, cost-effective, high transfection rate and easy operation.
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M3 - Conference contribution
AN - SCOPUS:33846906958
SN - 0780387406
SN - 9780780387409
T3 - Annual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings
SP - 522
EP - 525
BT - Proceedings of the 2005 27th Annual International Conference of the Engineering in Medicine and Biology Society, IEEE-EMBS 2005
T2 - 2005 27th Annual International Conference of the Engineering in Medicine and Biology Society, IEEE-EMBS 2005
Y2 - 1 September 2005 through 4 September 2005
ER -