Enhancement of bone marrow-derived mesenchymal stem cell osteogenesis and new bone formation in rats by obtusilactone A

Yi Hsiung Lin, Chung Yi Chen, Liang Yin Chou, Chung Hwan Chen, Lin Kang, Chau Zen Wang

Research output: Contribution to journalArticle

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Abstract

The natural pure compound obtusilactone A (OA) was identified in Cinnamomum kotoense Kanehira & Sasaki, and shows effective anti-cancer activity. We studied the effect of OA on osteogenesis of bone marrow-derived mesenchymal stem cells (BMSCs). OA possesses biocompatibility, stimulates Alkaline Phosphatase (ALP) activity and facilitates mineralization of BMSCs. Expression of osteogenesis markers BMP2, Runx2, Collagen I, and Osteocalcin was enhanced in OA-treated BMSCs. An in vivo rat model with local administration of OA via needle implantation to bone marrow-residing BMSCs revealed that OA increased the new bone formation and trabecular bone volume in tibias. Micro-CT images and H&E staining showed more trabecular bone at the needle-implanted site in the OA group than the normal saline group. Thus, OA confers an osteoinductive effect on BMSCs via induction of osteogenic marker gene expression, such as BMP2 and Runx2 expression and subsequently elevates ALP activity and mineralization, followed by enhanced trabecular bone formation in rat tibias. Therefore, OA is a potential osteoinductive drug to stimulate new bone formation by BMSCs.

Original languageEnglish
Article number2422
JournalInternational journal of molecular sciences
Volume18
Issue number11
DOIs
Publication statusPublished - 2017 Nov 15

Fingerprint

osteogenesis
bone marrow
stem cells
Stem cells
Mesenchymal Stromal Cells
Osteogenesis
rats
Rats
Bone
Bone Marrow
augmentation
tibia
phosphatases
needles
markers
bones
osteocalcin
Tibia
Needles
Alkaline Phosphatase

All Science Journal Classification (ASJC) codes

  • Catalysis
  • Molecular Biology
  • Spectroscopy
  • Computer Science Applications
  • Physical and Theoretical Chemistry
  • Organic Chemistry
  • Inorganic Chemistry

Cite this

Lin, Yi Hsiung ; Chen, Chung Yi ; Chou, Liang Yin ; Chen, Chung Hwan ; Kang, Lin ; Wang, Chau Zen. / Enhancement of bone marrow-derived mesenchymal stem cell osteogenesis and new bone formation in rats by obtusilactone A. In: International journal of molecular sciences. 2017 ; Vol. 18, No. 11.
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Enhancement of bone marrow-derived mesenchymal stem cell osteogenesis and new bone formation in rats by obtusilactone A. / Lin, Yi Hsiung; Chen, Chung Yi; Chou, Liang Yin; Chen, Chung Hwan; Kang, Lin; Wang, Chau Zen.

In: International journal of molecular sciences, Vol. 18, No. 11, 2422, 15.11.2017.

Research output: Contribution to journalArticle

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