Enhancement of C-phycocyanin purity using negative chromatography with chitosan-modified nanofiber membrane

I-Son Ng, Malcolm S.Y. Tang, Pau Loke Show, Zih Ming Chiou, Jung Chin Tsai, Yu Kaung Chang

Research output: Contribution to journalArticle

Abstract

In this work, a chitosan-modified nanofiber membrane was fabricated and used to examine the permeation characteristics of C-phycocyanin (CPC) obtained from Spirulina platensis. The effects of NaCl concentration (0.1–1.0 M), chitosan coupling pH (6–8), chitosan coupling concentration (0.1–3.0%), algal solution pH (6–8), algal mass concentration (0.1–1.0% dw/v), and membrane flux (4.08 × 10 −2 –2.04 × 10 −1 mL/min·cm 2 ) on the penetration performance of the membrane for CPC were investigated. The results show that the order of binding selectivity of the membrane for these proteins is contaminating proteins (TP) > allophycocyanin (APC) > CPC. TP and APC molecules were more easily adsorbed by the chitosan-modified membrane, and the CPC molecules most easily penetrated the membrane without being adsorbed, enhancing CPC purity. The purification factor and total mass flux were 3.3 fold and 66%, respectively, in a single step.

Original languageEnglish
Pages (from-to)615-628
Number of pages14
JournalInternational Journal of Biological Macromolecules
Volume132
DOIs
Publication statusPublished - 2019 Jul 1

Fingerprint

Phycocyanin
Nanofibers
Chitosan
Chromatography
Membranes
Spirulina
Molecules
Proteins
Permeation
Purification
Membrane Proteins
Mass transfer
Membrane
Enhancement
Fluxes

All Science Journal Classification (ASJC) codes

  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Economics and Econometrics
  • Energy(all)

Cite this

Ng, I-Son ; Tang, Malcolm S.Y. ; Show, Pau Loke ; Chiou, Zih Ming ; Tsai, Jung Chin ; Chang, Yu Kaung. / Enhancement of C-phycocyanin purity using negative chromatography with chitosan-modified nanofiber membrane. In: International Journal of Biological Macromolecules. 2019 ; Vol. 132. pp. 615-628.
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Enhancement of C-phycocyanin purity using negative chromatography with chitosan-modified nanofiber membrane. / Ng, I-Son; Tang, Malcolm S.Y.; Show, Pau Loke; Chiou, Zih Ming; Tsai, Jung Chin; Chang, Yu Kaung.

In: International Journal of Biological Macromolecules, Vol. 132, 01.07.2019, p. 615-628.

Research output: Contribution to journalArticle

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AU - Chang, Yu Kaung

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