Enhancement of humoral and cellular immune responses by an oral Salmonella choleraesuis vaccine expressing porcine prothymosin α

Ai-Li Shiau, Chung Cheng Chen, Yi Te Yo, Chun Yen Chu, Shiang Yiu Wang, Chao-Liang Wu

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Previously, we showed that murine prothymosin α (ProT) enhances the efficacy of a pseudorabies DNA vaccine delivered by bacterial vectors. In this study, we cloned and sequenced the cDNA for porcine ProT. The deduced amino acid sequence of porcine ProT exhibited high homology to ProT from other mammals. Oral Salmonella choleraesuis vaccine carrying the ProT eukaryotic expression plasmid protected mice against virulent S. choleraesuis challenge. The adjuvant effect of ProT on humoral and cellular immune responses enhanced protective efficacy of the vaccine. Furthermore, both humoral and cellular immune responses played roles in the protective immune responses induced by the vaccine. Collectively, our results show that delivery of the ProT gene carried by attenuated S. choleraesuis augmented the immunogenicity of oral S. choleraesuis vaccine.

Original languageEnglish
Pages (from-to)5563-5571
Number of pages9
JournalVaccine
Volume23
Issue number48-49
DOIs
Publication statusPublished - 2005 Dec 1

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Salmonella Vaccines
Salmonella enterica
Humoral Immunity
humoral immunity
Cellular Immunity
cell-mediated immunity
mouth
Swine
Vaccines
vaccines
Pseudorabies Vaccines
swine
DNA Vaccines
immune response
Aujeszky disease
Mammals
Amino Acid Sequence
mice
Plasmids
recombinant vaccines

All Science Journal Classification (ASJC) codes

  • Immunology
  • Microbiology
  • Virology
  • Infectious Diseases
  • Public Health, Environmental and Occupational Health
  • veterinary(all)

Cite this

Shiau, Ai-Li ; Chen, Chung Cheng ; Yo, Yi Te ; Chu, Chun Yen ; Wang, Shiang Yiu ; Wu, Chao-Liang. / Enhancement of humoral and cellular immune responses by an oral Salmonella choleraesuis vaccine expressing porcine prothymosin α. In: Vaccine. 2005 ; Vol. 23, No. 48-49. pp. 5563-5571.
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Enhancement of humoral and cellular immune responses by an oral Salmonella choleraesuis vaccine expressing porcine prothymosin α. / Shiau, Ai-Li; Chen, Chung Cheng; Yo, Yi Te; Chu, Chun Yen; Wang, Shiang Yiu; Wu, Chao-Liang.

In: Vaccine, Vol. 23, No. 48-49, 01.12.2005, p. 5563-5571.

Research output: Contribution to journalArticle

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