Enhancement of NMDA receptor-mediated synaptic potential by isoproterenol is blocked by Rp-adenosine 3′,5′-cyclic monophosphothioate

Chiung Chun Huang, Jing Jane Tsai, Po Wu Gean

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

The intracellular mechanisms underlying the facilitatory action of isoproterenol (Iso) on the NMDA receptor-mediated synaptic potential (EPSPnmda) was investigated in an in vitro slice preparation of rat amygdala. Intracellular recordings were made from basolateral amygdala neurons in the presence of 6-cyano-7-nitroquinoxaline-2,3-dione (CNQX, 10 μM) and picrotoxin (50 μM) which block non-NMDA and GABAa receptors, respectively. Superfusion of Iso (15 μM) produced a sustained increase in EPSPnmda. Rp-adenosine-3′,5′-cyclic monophosphothioate (Rp-cAMPS), a potent inhibitor of protein kinase A (PKA) alone decreased the amplitude of EPSPnmda below baseline values and prevented the subsequent potentiation by Iso. Forskolin, a direct activator of adenylate cyclase, mimics the effect of Iso, and Rp-cAMPS also reversed forskolin-induced enhancement of EPSPnmda. These results suggest that cAMP-dependent protein kinase mediates the enhancement of EPSPnmda by Iso in the amygdala.

Original languageEnglish
Pages (from-to)207-210
Number of pages4
JournalNeuroscience Letters
Volume161
Issue number2
DOIs
Publication statusPublished - 1993 Oct 29

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)

Fingerprint Dive into the research topics of 'Enhancement of NMDA receptor-mediated synaptic potential by isoproterenol is blocked by Rp-adenosine 3′,5′-cyclic monophosphothioate'. Together they form a unique fingerprint.

  • Cite this