Enhancement of TNF-α expression does not trigger apoptosis upon exposure of glial cells to lead and lipopolysaccharide

Yu Jung Cheng, Bei-Chang Yang, Wen Chuan Hsieh, Bu-Miin Huang, Ming-Yi Liu

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Lead (Pb) and lipopolysaccharide (LPS) affect not only neurons but also glial cells, and cause neurological impairment in the brain. We have previously shown an enhancement of tumor necrosis factor-α (TNF-α) in U-373MG glial cells after exposure of Pb and LPS. To determine whether Pb- or LPS-induced TNF-α triggers glial cell death, the apoptotic response was investigated in glial cells both in vitro and in vivo. In vitro, Pb ranging from 0.1 to 10 μM increased the expression of TNF-α in a dose-dependent manner in U-373MG cells. Similarly, LPS (500 ng/ml) stimulated TNF-α expression in the same cells. A combination of LPS and Pb did not cause higher mRNA TNF-α expression than LPS or Pb alone. However, exposure to Pb and LPS did not affect apoptosis in U-373MG cells detected by flow cytometric analysis with merocyanine 540 staining and propidium idodine staining. In B6 mice, glial cells in the brain were immunostained positive for TNF-α after an intraperitoncal administration of Pb (12.5 mg/kg) or LPS (10 mg/kg). Consistent with the findings in the cell culture system, few apoptotic cells were detected in glial and neurons by terminal deoxynucleotidyl transferase (TdT) -mediated dUTP nick end-labeling (TUNEL) in the brain of B6 mice. We conclude that induction of TNF-α alone by Pb and LPS does not cause cell apoptosis in cells of glial origin.

Original languageEnglish
Pages (from-to)183-191
Number of pages9
JournalToxicology
Volume178
Issue number3
DOIs
Publication statusPublished - 2002 Sep 16

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Neuroglia
Lipopolysaccharides
Tumor Necrosis Factor-alpha
Apoptosis
Brain
Neurons
Staining and Labeling
DNA Nucleotidylexotransferase
Propidium
Cell death
Cell culture
Labeling
Cell Death
Cell Culture Techniques
Cells
Messenger RNA

All Science Journal Classification (ASJC) codes

  • Toxicology

Cite this

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title = "Enhancement of TNF-α expression does not trigger apoptosis upon exposure of glial cells to lead and lipopolysaccharide",
abstract = "Lead (Pb) and lipopolysaccharide (LPS) affect not only neurons but also glial cells, and cause neurological impairment in the brain. We have previously shown an enhancement of tumor necrosis factor-α (TNF-α) in U-373MG glial cells after exposure of Pb and LPS. To determine whether Pb- or LPS-induced TNF-α triggers glial cell death, the apoptotic response was investigated in glial cells both in vitro and in vivo. In vitro, Pb ranging from 0.1 to 10 μM increased the expression of TNF-α in a dose-dependent manner in U-373MG cells. Similarly, LPS (500 ng/ml) stimulated TNF-α expression in the same cells. A combination of LPS and Pb did not cause higher mRNA TNF-α expression than LPS or Pb alone. However, exposure to Pb and LPS did not affect apoptosis in U-373MG cells detected by flow cytometric analysis with merocyanine 540 staining and propidium idodine staining. In B6 mice, glial cells in the brain were immunostained positive for TNF-α after an intraperitoncal administration of Pb (12.5 mg/kg) or LPS (10 mg/kg). Consistent with the findings in the cell culture system, few apoptotic cells were detected in glial and neurons by terminal deoxynucleotidyl transferase (TdT) -mediated dUTP nick end-labeling (TUNEL) in the brain of B6 mice. We conclude that induction of TNF-α alone by Pb and LPS does not cause cell apoptosis in cells of glial origin.",
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Enhancement of TNF-α expression does not trigger apoptosis upon exposure of glial cells to lead and lipopolysaccharide. / Cheng, Yu Jung; Yang, Bei-Chang; Hsieh, Wen Chuan; Huang, Bu-Miin; Liu, Ming-Yi.

In: Toxicology, Vol. 178, No. 3, 16.09.2002, p. 183-191.

Research output: Contribution to journalArticle

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AU - Cheng, Yu Jung

AU - Yang, Bei-Chang

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