Enhanching biohydrogen production from Chlorella vulgaris FSP-E under mixotrophic cultivation conditions

Chun Yen Chen, Ching Yu Chang, Yung Chung Lo, Shih Hsin Ho, Jo Shu Chang

Research output: Contribution to journalConference articlepeer-review

8 Citations (Scopus)


In this study, three indigenous microalgae strains (Scenedesmus subspicatus GY-16, Chlorella vulgaris FSP-E, and Anistrodesmus gracilis GY-09) were evaluated for their performance on producing carbohydrates, which serve as feedstock for bioH2 production. The results show that C. vulgaris FSP-E displayed the highest growth rate (825.6 mg/L/d) and carbohydrate productivity (365.8 mg/L/d). Different sodium acetate concentrations were supplemented into the growth medium to investigate the effect of organic carbon source on biomass and carbohydrate productivity. The results show that using 2000 mg/l sodium acetate to grow C. vulgaris FSP-E resulted in the highest biomass and carbohydrate productivity of 1022.3 mg/l/d and 498.5 mg/l/d, respectively. Meanwhile, to assess the practical applicability of the proposed microalgae-based carbohydrates production system, the photobioreactor was further operated on semi-batch mode for a prolonged incubation time under the optimal conditions. The results show that the biomass and carbohydrate productivity of C. vulgaris FSP-E was stably maintained at 1173.6 and 371.4 mg/L/d, respectively. Due to the high carbohydrate content, C. vulgaris FSP-E is considered an excellent feedstock for biohydrogen fermentation. Using the acidic hydrolysate of C. vulgaris FSP-E as feedstock, the separate hydrolysis and fermentation (SHF) process could achieve high-yield hydrogen production with a maximum hydrogen yield of 2.67 g hydrogen/g microalgae biomass, which is higher than most reported values. These results indicate that using carbohydrate-rich microalgae as feedstock to produce biohydrogen is indeed feasible for practical applications.

Original languageEnglish
Pages (from-to)870-873
Number of pages4
JournalEnergy Procedia
Publication statusPublished - 2014
Event6th International Conference on Applied Energy, ICAE 2014 - Taipei, Taiwan
Duration: 2014 May 302014 Jun 2

All Science Journal Classification (ASJC) codes

  • General Energy


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