Enhancing butanol production with Clostridium pasteurianum CH4 using sequential glucose-glycerol addition and simultaneous dual-substrate cultivation strategies

Wei Chen Kao, De Shun Lin, Chieh Lun Cheng, Bor Yann Chen, Chiu Yue Lin, Jo Shu Chang

Research output: Contribution to journalArticlepeer-review

34 Citations (Scopus)

Abstract

Adding butyrate significantly enhanced butanol production from glycerol with Clostridium pasteurianum CH4, which predominantly produces butyrate (instead of butanol) when grown on glucose. Hence, the butyrate produced from assimilating glucose can be used to stimulate butanol production from glycerol under dual-substrate cultivation with glucose and glycerol. This proposed butanol production process was conducted by employing sequential or simultaneous addition of the two substrates. The latter approach exhibited better carbon source utilization and butanol production efficiencies. Under the optimal glucose to glycerol ratio (20gL-1 to 60gL-1), the simultaneous dual-substrate strategy obtained maximum butanol titer, productivity and yield of 13.3gL-1, 0.28gL-1h-1, and 0.38mol butanol/mol glycerol, respectively. Moreover, bagasse and crude glycerol as dual-substrates were also converted into butanol efficiently with a maximum butanol concentration, productivity and yield of 11.8gL-1, 0.14gL-1h-1, and 0.33mol butanol/mol glycerol, respectively.

Original languageEnglish
Pages (from-to)324-330
Number of pages7
JournalBioresource technology
Volume135
DOIs
Publication statusPublished - 2013 May

All Science Journal Classification (ASJC) codes

  • Bioengineering
  • Environmental Engineering
  • Renewable Energy, Sustainability and the Environment
  • Waste Management and Disposal

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