Enhancing lutein production with Chlorella sorokiniana Mb-1 by optimizing acetate and nitrate concentrations under mixotrophic growth

Chun Yen Chen, Shih Hsin Ho, Chen Chun Liu, Jo Shu Chang

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10 Citations (Scopus)

Abstract

Lutein is a xanthophyll pigment known for its antioxidant activities and its protective role against eye damage. In this study, lutein production from microalgae with mixotrophic cultivation is demonstrated. Four acetate-utilizing microalgae with high lutein accumulating ability were isolated and tested for lutein production. Chlorella sorokiniana Mb-1, with the highest lutein productivity, was chosen for further studies. The effects of acetate and nitrate concentrations in the BG11-based medium were examined. The optimum concentrations of nitrate and acetate were determined using a statistical experimental design methodology. The results show that the optimal composition of acetate and nitrate was 4.88 g/L and 1.83 g/L, respectively, leading to a predicted lutein productivity of 3.96 mg/L/day. This predicted value was verified by experiments indicating that lutein productivity could be enhanced from 2.79 mg/L/day to 3.97 mg/L/day (42% enhancement) via optimizing the carbon and nitrogen sources in the mixotrophic culture medium.

Original languageEnglish
Pages (from-to)88-96
Number of pages9
JournalJournal of the Taiwan Institute of Chemical Engineers
Volume79
DOIs
Publication statusPublished - 2017 Oct

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Lutein
Nitrates
Acetates
Productivity
Antioxidants
Pigments
Design of experiments
Nitrogen
Xanthophylls
Carbon
Chemical analysis
Culture Media
Experiments

All Science Journal Classification (ASJC) codes

  • Chemistry(all)
  • Chemical Engineering(all)

Cite this

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title = "Enhancing lutein production with Chlorella sorokiniana Mb-1 by optimizing acetate and nitrate concentrations under mixotrophic growth",
abstract = "Lutein is a xanthophyll pigment known for its antioxidant activities and its protective role against eye damage. In this study, lutein production from microalgae with mixotrophic cultivation is demonstrated. Four acetate-utilizing microalgae with high lutein accumulating ability were isolated and tested for lutein production. Chlorella sorokiniana Mb-1, with the highest lutein productivity, was chosen for further studies. The effects of acetate and nitrate concentrations in the BG11-based medium were examined. The optimum concentrations of nitrate and acetate were determined using a statistical experimental design methodology. The results show that the optimal composition of acetate and nitrate was 4.88 g/L and 1.83 g/L, respectively, leading to a predicted lutein productivity of 3.96 mg/L/day. This predicted value was verified by experiments indicating that lutein productivity could be enhanced from 2.79 mg/L/day to 3.97 mg/L/day (42{\%} enhancement) via optimizing the carbon and nitrogen sources in the mixotrophic culture medium.",
author = "Chen, {Chun Yen} and Ho, {Shih Hsin} and Liu, {Chen Chun} and Chang, {Jo Shu}",
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T1 - Enhancing lutein production with Chlorella sorokiniana Mb-1 by optimizing acetate and nitrate concentrations under mixotrophic growth

AU - Chen, Chun Yen

AU - Ho, Shih Hsin

AU - Liu, Chen Chun

AU - Chang, Jo Shu

PY - 2017/10

Y1 - 2017/10

N2 - Lutein is a xanthophyll pigment known for its antioxidant activities and its protective role against eye damage. In this study, lutein production from microalgae with mixotrophic cultivation is demonstrated. Four acetate-utilizing microalgae with high lutein accumulating ability were isolated and tested for lutein production. Chlorella sorokiniana Mb-1, with the highest lutein productivity, was chosen for further studies. The effects of acetate and nitrate concentrations in the BG11-based medium were examined. The optimum concentrations of nitrate and acetate were determined using a statistical experimental design methodology. The results show that the optimal composition of acetate and nitrate was 4.88 g/L and 1.83 g/L, respectively, leading to a predicted lutein productivity of 3.96 mg/L/day. This predicted value was verified by experiments indicating that lutein productivity could be enhanced from 2.79 mg/L/day to 3.97 mg/L/day (42% enhancement) via optimizing the carbon and nitrogen sources in the mixotrophic culture medium.

AB - Lutein is a xanthophyll pigment known for its antioxidant activities and its protective role against eye damage. In this study, lutein production from microalgae with mixotrophic cultivation is demonstrated. Four acetate-utilizing microalgae with high lutein accumulating ability were isolated and tested for lutein production. Chlorella sorokiniana Mb-1, with the highest lutein productivity, was chosen for further studies. The effects of acetate and nitrate concentrations in the BG11-based medium were examined. The optimum concentrations of nitrate and acetate were determined using a statistical experimental design methodology. The results show that the optimal composition of acetate and nitrate was 4.88 g/L and 1.83 g/L, respectively, leading to a predicted lutein productivity of 3.96 mg/L/day. This predicted value was verified by experiments indicating that lutein productivity could be enhanced from 2.79 mg/L/day to 3.97 mg/L/day (42% enhancement) via optimizing the carbon and nitrogen sources in the mixotrophic culture medium.

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