TY - JOUR
T1 - Enhancing phototropic hydrogen production by solid-carrier assisted fermentation and internal optical-fiber illumination
AU - Chen, Chun Yen
AU - Chang, Jo Shu
N1 - Funding Information:
The authors gratefully acknowledge financial supports from Taiwan's National Science Council (Grant Nos. NSC-93-2211-E-006-040 and NSC 94-2211-E-006-026) as well as Taiwan's Bureau of Energy (Grant Nos. 93-ET-7-006-001-ET and 94-ET-7-006-004-ET). We also thank Prof. C. M. Lee at National Chung Hsing University for providing the bacterial strain used in this study.
PY - 2006/9
Y1 - 2006/9
N2 - Three strategies were applied to promote the phototrphic H2 production of an indigenous purple nonsulfur bacterium Rhodopseudomonas palustris WP3-5 using acetate as the sole carbon substrate. First, a small amount of solid carriers (e.g., activated carbon, silica gel, and clay) was supplemented to fermentation broth to stimulate cell growth and H2 production. Second, the acetate concentration leading to optimal production of H2 was identified. Finally, an innovative optical-fiber illuminating system was designed to facilitate the efficiency of the photobioreactor. The results show that addition of clay and silica gel was effective in promoting H2 production, resulting in 67.2-50.9% and 37.2-32.5% increases in H2 production rate (vH2) and H2 yield (YH2), respectively. For clay-supplemented batch cultures, the optimal acetate concentration was 1000 mg COD/l, leading to a vH2 and YH2 value of 28.5 ml/h/l and 2.97 mol H2/mol acetate, respectively. Moreover, combination of internal optical-fiber illumination system, clay addition, and optimal acetate concentration further elevated the vH2 and YH2 to a maximum level of 43.8 ml/h/l and 3.63 mol H2/mol acetate, respectively. These values are considerably higher than most reported results from relevant studies. Meanwhile, the results of continuous cultures operated at 36 h HRT (hydraulic retention time) show that the high phototrophic H2 production efficiency was stably maintained for over 17 days with a steady-state vH2 and YH2 of 44.0 ml/h/l and 3.57 mol H2/mol acetate, respectively.
AB - Three strategies were applied to promote the phototrphic H2 production of an indigenous purple nonsulfur bacterium Rhodopseudomonas palustris WP3-5 using acetate as the sole carbon substrate. First, a small amount of solid carriers (e.g., activated carbon, silica gel, and clay) was supplemented to fermentation broth to stimulate cell growth and H2 production. Second, the acetate concentration leading to optimal production of H2 was identified. Finally, an innovative optical-fiber illuminating system was designed to facilitate the efficiency of the photobioreactor. The results show that addition of clay and silica gel was effective in promoting H2 production, resulting in 67.2-50.9% and 37.2-32.5% increases in H2 production rate (vH2) and H2 yield (YH2), respectively. For clay-supplemented batch cultures, the optimal acetate concentration was 1000 mg COD/l, leading to a vH2 and YH2 value of 28.5 ml/h/l and 2.97 mol H2/mol acetate, respectively. Moreover, combination of internal optical-fiber illumination system, clay addition, and optimal acetate concentration further elevated the vH2 and YH2 to a maximum level of 43.8 ml/h/l and 3.63 mol H2/mol acetate, respectively. These values are considerably higher than most reported results from relevant studies. Meanwhile, the results of continuous cultures operated at 36 h HRT (hydraulic retention time) show that the high phototrophic H2 production efficiency was stably maintained for over 17 days with a steady-state vH2 and YH2 of 44.0 ml/h/l and 3.57 mol H2/mol acetate, respectively.
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U2 - 10.1016/j.procbio.2006.05.005
DO - 10.1016/j.procbio.2006.05.005
M3 - Article
AN - SCOPUS:33746771022
VL - 41
SP - 2041
EP - 2049
JO - Process Biochemistry
JF - Process Biochemistry
SN - 0032-9592
IS - 9
ER -