Enhancing production of lutein by a mixotrophic cultivation system using microalga Scenedesmus obliquus CWL-1

Wei Chuan Chen, Yin Che Hsu, Jo Shu Chang, Shih Hsin Ho, Li Fen Wang, Yu Hong Wei

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

This work studies a series of strategies in the production of lutein by Scenedesmus obliquus CWL-1 under mixotrophic cultivation. Our experimental results revealed that the optimal conditions associated with light-related strategies were 12 h light period followed by a 12 h dark period and blue to red light under mixotrophic cultivation. Under such conditions, the biomass, lutein content and lutein productivity were maximized to 9.88 (g/L), 1.78 (mg/g) and 1.43 (mg/L/day), respectively. Moreover, the assimilation of 4.5 g/L of calcium nitrate into S. obliquus CWL-1 increased the maximal biomass (12.73 g/L) and the highest maximal lutein productivity (3.06 mg/L/day), while the assimilation of 1.5 g/L of calcium nitrate yielded the highest maximal lutein content of 2.45 mg/g. The highest maximal lutein productivity of 4.96 (mg/L/day) was obtained when fed-batch fermentation was conducted, and this value was approximately 11-folds that obtained using the batch system.

Original languageEnglish
Article number121891
JournalBioresource technology
Volume291
DOIs
Publication statusPublished - 2019 Nov

Fingerprint

Lutein
microalga
Productivity
productivity
Calcium
Nitrates
Biomass
calcium
nitrate
biomass
Fermentation
fermentation
fold
cultivation system
assimilation

All Science Journal Classification (ASJC) codes

  • Bioengineering
  • Environmental Engineering
  • Renewable Energy, Sustainability and the Environment
  • Waste Management and Disposal

Cite this

Chen, Wei Chuan ; Hsu, Yin Che ; Chang, Jo Shu ; Ho, Shih Hsin ; Wang, Li Fen ; Wei, Yu Hong. / Enhancing production of lutein by a mixotrophic cultivation system using microalga Scenedesmus obliquus CWL-1. In: Bioresource technology. 2019 ; Vol. 291.
@article{f68b3d92ebaf43aa90f4a7fef1b4c1f0,
title = "Enhancing production of lutein by a mixotrophic cultivation system using microalga Scenedesmus obliquus CWL-1",
abstract = "This work studies a series of strategies in the production of lutein by Scenedesmus obliquus CWL-1 under mixotrophic cultivation. Our experimental results revealed that the optimal conditions associated with light-related strategies were 12 h light period followed by a 12 h dark period and blue to red light under mixotrophic cultivation. Under such conditions, the biomass, lutein content and lutein productivity were maximized to 9.88 (g/L), 1.78 (mg/g) and 1.43 (mg/L/day), respectively. Moreover, the assimilation of 4.5 g/L of calcium nitrate into S. obliquus CWL-1 increased the maximal biomass (12.73 g/L) and the highest maximal lutein productivity (3.06 mg/L/day), while the assimilation of 1.5 g/L of calcium nitrate yielded the highest maximal lutein content of 2.45 mg/g. The highest maximal lutein productivity of 4.96 (mg/L/day) was obtained when fed-batch fermentation was conducted, and this value was approximately 11-folds that obtained using the batch system.",
author = "Chen, {Wei Chuan} and Hsu, {Yin Che} and Chang, {Jo Shu} and Ho, {Shih Hsin} and Wang, {Li Fen} and Wei, {Yu Hong}",
year = "2019",
month = "11",
doi = "10.1016/j.biortech.2019.121891",
language = "English",
volume = "291",
journal = "Bioresource Technology",
issn = "0960-8524",
publisher = "Elsevier Limited",

}

Enhancing production of lutein by a mixotrophic cultivation system using microalga Scenedesmus obliquus CWL-1. / Chen, Wei Chuan; Hsu, Yin Che; Chang, Jo Shu; Ho, Shih Hsin; Wang, Li Fen; Wei, Yu Hong.

In: Bioresource technology, Vol. 291, 121891, 11.2019.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Enhancing production of lutein by a mixotrophic cultivation system using microalga Scenedesmus obliquus CWL-1

AU - Chen, Wei Chuan

AU - Hsu, Yin Che

AU - Chang, Jo Shu

AU - Ho, Shih Hsin

AU - Wang, Li Fen

AU - Wei, Yu Hong

PY - 2019/11

Y1 - 2019/11

N2 - This work studies a series of strategies in the production of lutein by Scenedesmus obliquus CWL-1 under mixotrophic cultivation. Our experimental results revealed that the optimal conditions associated with light-related strategies were 12 h light period followed by a 12 h dark period and blue to red light under mixotrophic cultivation. Under such conditions, the biomass, lutein content and lutein productivity were maximized to 9.88 (g/L), 1.78 (mg/g) and 1.43 (mg/L/day), respectively. Moreover, the assimilation of 4.5 g/L of calcium nitrate into S. obliquus CWL-1 increased the maximal biomass (12.73 g/L) and the highest maximal lutein productivity (3.06 mg/L/day), while the assimilation of 1.5 g/L of calcium nitrate yielded the highest maximal lutein content of 2.45 mg/g. The highest maximal lutein productivity of 4.96 (mg/L/day) was obtained when fed-batch fermentation was conducted, and this value was approximately 11-folds that obtained using the batch system.

AB - This work studies a series of strategies in the production of lutein by Scenedesmus obliquus CWL-1 under mixotrophic cultivation. Our experimental results revealed that the optimal conditions associated with light-related strategies were 12 h light period followed by a 12 h dark period and blue to red light under mixotrophic cultivation. Under such conditions, the biomass, lutein content and lutein productivity were maximized to 9.88 (g/L), 1.78 (mg/g) and 1.43 (mg/L/day), respectively. Moreover, the assimilation of 4.5 g/L of calcium nitrate into S. obliquus CWL-1 increased the maximal biomass (12.73 g/L) and the highest maximal lutein productivity (3.06 mg/L/day), while the assimilation of 1.5 g/L of calcium nitrate yielded the highest maximal lutein content of 2.45 mg/g. The highest maximal lutein productivity of 4.96 (mg/L/day) was obtained when fed-batch fermentation was conducted, and this value was approximately 11-folds that obtained using the batch system.

UR - http://www.scopus.com/inward/record.url?scp=85071280996&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85071280996&partnerID=8YFLogxK

U2 - 10.1016/j.biortech.2019.121891

DO - 10.1016/j.biortech.2019.121891

M3 - Article

C2 - 31387049

AN - SCOPUS:85071280996

VL - 291

JO - Bioresource Technology

JF - Bioresource Technology

SN - 0960-8524

M1 - 121891

ER -