Environmental pH changes, but not the LuxS signalling pathway, regulate SpeB expression in M1 group A streptococci

Chuan Chiang-Ni, Po Xing Zheng, Pei-Jane Tsai, Woei-Jer Chuang, Yee-Shin Lin, Ching-Chuan Liu, Jiunn Jong Wu

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

The autoinducer-2/LuxS signalling pathway participates in quorum sensing in diverse bacterial species. In group A streptococci (GAS), LuxS has been shown to be involved in regulating the expression of several important virulence factors. Streptococcal pyrogenic exotoxin B (SpeB), a cysteine protease that has important roles in GAS pathogenesis, is positively regulated by LuxS in M3 and M5 strains. In the present study, it was found that the supernatant harvested from an overnight culture stimulated M1 strains to express speB. However, mutation of the luxS gene in M1 strains or treating M1 strains with luxS mutant culture supernatant did not affect speB expression, indicating that the LuxS pathway is not involved in regulation of speB expression in M1 strains. In addition, the acid property of culture broth was found to be able to stimulate M1 strains to express speB in the same LuxS-independent manner. These results indicate that speB expression in M1 strains is induced by environmental pH changes but is not regulated by the LuxS signalling pathway.

Original languageEnglish
Pages (from-to)16-22
Number of pages7
JournalJournal of Medical Microbiology
Volume61
Issue number1
DOIs
Publication statusPublished - 2012 Jan 1

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Streptococcus
Quorum Sensing
Cysteine Proteases
Virulence Factors
Mutation
Acids
Genes
erythrogenic toxin
N-octanoylhomoserine lactone

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Microbiology (medical)

Cite this

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title = "Environmental pH changes, but not the LuxS signalling pathway, regulate SpeB expression in M1 group A streptococci",
abstract = "The autoinducer-2/LuxS signalling pathway participates in quorum sensing in diverse bacterial species. In group A streptococci (GAS), LuxS has been shown to be involved in regulating the expression of several important virulence factors. Streptococcal pyrogenic exotoxin B (SpeB), a cysteine protease that has important roles in GAS pathogenesis, is positively regulated by LuxS in M3 and M5 strains. In the present study, it was found that the supernatant harvested from an overnight culture stimulated M1 strains to express speB. However, mutation of the luxS gene in M1 strains or treating M1 strains with luxS mutant culture supernatant did not affect speB expression, indicating that the LuxS pathway is not involved in regulation of speB expression in M1 strains. In addition, the acid property of culture broth was found to be able to stimulate M1 strains to express speB in the same LuxS-independent manner. These results indicate that speB expression in M1 strains is induced by environmental pH changes but is not regulated by the LuxS signalling pathway.",
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Environmental pH changes, but not the LuxS signalling pathway, regulate SpeB expression in M1 group A streptococci. / Chiang-Ni, Chuan; Zheng, Po Xing; Tsai, Pei-Jane; Chuang, Woei-Jer; Lin, Yee-Shin; Liu, Ching-Chuan; Wu, Jiunn Jong.

In: Journal of Medical Microbiology, Vol. 61, No. 1, 01.01.2012, p. 16-22.

Research output: Contribution to journalArticle

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AU - Chiang-Ni, Chuan

AU - Zheng, Po Xing

AU - Tsai, Pei-Jane

AU - Chuang, Woei-Jer

AU - Lin, Yee-Shin

AU - Liu, Ching-Chuan

AU - Wu, Jiunn Jong

PY - 2012/1/1

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