Enzymatic treatment of lignocellulosic wastes for anaerobic digestion and bioenergy production

Ganesh D. Saratale, Liang Jung Chien, Jo-Shu Chang

Research output: Chapter in Book/Report/Conference proceedingChapter

14 Citations (Scopus)


Active research has been conducted on the production of biofuels from the abundant and inexpensive lignocellulosic wastes of forestry, agriculture, and municipal solid wastes. However, degradation of lignocellulose is hindered by the recalcitrant nature of lignocellulose. Hence, hydrolysis/saccharification of lignocellulose becomes the rate-limiting step for the fermentative production of cellulosic biofuels (such as H2 and ethanol). Hydrolysis of cellulosic materials by biological means is environmentally benign and could be achieved either by using cellulolytic microorganisms or cellulolytic enzymes collected from those microorganisms. Biofuels production could be achieved by direct fermentation of raw lignocellulosic wastes or by a twostage process, in which the hydrolysis step and the anaerobic fermentation step are operated separately. In this chapter, we review the state-of-the-art of the following aspects related to the enzymatic hydrolysis of lignocellulosic wastes for anaerobic fermentation and bioenergy production: (1) structure of plant cell walls and their cellulose, hemicellulose, and lignin components; (2) lignocellulose-degrading microorganisms and their characterisitics; (3) production of enzymes degrading lignocellulose; (4) treatment of wastes using lignocellulose-degrading enzymes; and (5) anaerobic fermentation process for bioenergy production from enzymatically pretreated lignocellusic wastes.

Original languageEnglish
Title of host publicationEnvironmental Anaerobic Technology
Subtitle of host publicationApplications and New Developments
PublisherImperial College Press
Number of pages30
ISBN (Electronic)9781848165434
ISBN (Print)9781848165427
Publication statusPublished - 2010 Jan 1

All Science Journal Classification (ASJC) codes

  • General Biochemistry,Genetics and Molecular Biology
  • General Chemical Engineering
  • General Chemistry
  • General Engineering
  • General Environmental Science


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