(−)-Epigallocatechin-3-gallate (EGCG) enhances healing of femoral bone defect

Sung Yen Lin, Lin Kang, Jian Chih Chen, Chau Zen Wang, Han Hsiang Huang, Mon Juan Lee, Tsung Lin Cheng, Chi Fen Chang, Yi Shan Lin, Chung Hwan Chen

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Background: Previously, we found that (−)-epigallocatechin-3-gallate (EGCG) enhanced osteogenic differentiation of murine bone marrow mesenchymal stem cells by increasing the mRNA expression of osteogenesis-related genes, alkaline phosphatase activity and eventually mineralization. We further found EGCG supplementation preserved bone mass and microarchitecture in female rats during estrogen deficiency in the proximal tibia and lumbar spine at least in part by increasing bone morphogenetic protein-2 (BMP2). BMP2 can enhance de novo bone formation. Purpose: In this study, we evaluate the effect of local EGCG application in de novo bone formation in bone defect healing. Methods: Twenty-four rats aged 4 months were weight-matched and randomly allocated to 2 groups: defect control with vehicle treatment (control) and defect with 10 µM EGCG treatment (EGCG). Daily vehicle and EGCG were applied locally by percutaneous local injection 2 days after defect creation for 2 weeks. Four weeks after treatment, animals were sacrificed for micro-computed tomography (μ-CT) and biomechanical analysis. Results: Local EGCG at femoral defect can enhance de novo bone formation by increasing bone volume and subsequently improve mechanical properties including max load, break point, stiffness, area under the max load curve, area under the break point curve and ultimate stress. Conclusions: Local EGCG may enhance bone defect healing via at least partly by the de novo bone formation of BMP-2.

Original languageEnglish
Pages (from-to)165-171
Number of pages7
JournalPhytomedicine
Volume55
DOIs
Publication statusPublished - 2019 Mar 1

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Thigh
Bone and Bones
Osteogenesis
Bone Morphogenetic Protein 2
epigallocatechin gallate
Tibia
Mesenchymal Stromal Cells
Area Under Curve
Alkaline Phosphatase
Estrogens
Spine
Therapeutics
Bone Marrow
Tomography
Weights and Measures
Control Groups
Messenger RNA
Injections
Genes

All Science Journal Classification (ASJC) codes

  • Molecular Medicine
  • Pharmacology
  • Pharmaceutical Science
  • Drug Discovery
  • Complementary and alternative medicine

Cite this

Lin, S. Y., Kang, L., Chen, J. C., Wang, C. Z., Huang, H. H., Lee, M. J., ... Chen, C. H. (2019). (−)-Epigallocatechin-3-gallate (EGCG) enhances healing of femoral bone defect. Phytomedicine, 55, 165-171. https://doi.org/10.1016/j.phymed.2018.07.012
Lin, Sung Yen ; Kang, Lin ; Chen, Jian Chih ; Wang, Chau Zen ; Huang, Han Hsiang ; Lee, Mon Juan ; Cheng, Tsung Lin ; Chang, Chi Fen ; Lin, Yi Shan ; Chen, Chung Hwan. / (−)-Epigallocatechin-3-gallate (EGCG) enhances healing of femoral bone defect. In: Phytomedicine. 2019 ; Vol. 55. pp. 165-171.
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abstract = "Background: Previously, we found that (−)-epigallocatechin-3-gallate (EGCG) enhanced osteogenic differentiation of murine bone marrow mesenchymal stem cells by increasing the mRNA expression of osteogenesis-related genes, alkaline phosphatase activity and eventually mineralization. We further found EGCG supplementation preserved bone mass and microarchitecture in female rats during estrogen deficiency in the proximal tibia and lumbar spine at least in part by increasing bone morphogenetic protein-2 (BMP2). BMP2 can enhance de novo bone formation. Purpose: In this study, we evaluate the effect of local EGCG application in de novo bone formation in bone defect healing. Methods: Twenty-four rats aged 4 months were weight-matched and randomly allocated to 2 groups: defect control with vehicle treatment (control) and defect with 10 µM EGCG treatment (EGCG). Daily vehicle and EGCG were applied locally by percutaneous local injection 2 days after defect creation for 2 weeks. Four weeks after treatment, animals were sacrificed for micro-computed tomography (μ-CT) and biomechanical analysis. Results: Local EGCG at femoral defect can enhance de novo bone formation by increasing bone volume and subsequently improve mechanical properties including max load, break point, stiffness, area under the max load curve, area under the break point curve and ultimate stress. Conclusions: Local EGCG may enhance bone defect healing via at least partly by the de novo bone formation of BMP-2.",
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Lin, SY, Kang, L, Chen, JC, Wang, CZ, Huang, HH, Lee, MJ, Cheng, TL, Chang, CF, Lin, YS & Chen, CH 2019, '(−)-Epigallocatechin-3-gallate (EGCG) enhances healing of femoral bone defect', Phytomedicine, vol. 55, pp. 165-171. https://doi.org/10.1016/j.phymed.2018.07.012

(−)-Epigallocatechin-3-gallate (EGCG) enhances healing of femoral bone defect. / Lin, Sung Yen; Kang, Lin; Chen, Jian Chih; Wang, Chau Zen; Huang, Han Hsiang; Lee, Mon Juan; Cheng, Tsung Lin; Chang, Chi Fen; Lin, Yi Shan; Chen, Chung Hwan.

In: Phytomedicine, Vol. 55, 01.03.2019, p. 165-171.

Research output: Contribution to journalArticle

TY - JOUR

T1 - (−)-Epigallocatechin-3-gallate (EGCG) enhances healing of femoral bone defect

AU - Lin, Sung Yen

AU - Kang, Lin

AU - Chen, Jian Chih

AU - Wang, Chau Zen

AU - Huang, Han Hsiang

AU - Lee, Mon Juan

AU - Cheng, Tsung Lin

AU - Chang, Chi Fen

AU - Lin, Yi Shan

AU - Chen, Chung Hwan

PY - 2019/3/1

Y1 - 2019/3/1

N2 - Background: Previously, we found that (−)-epigallocatechin-3-gallate (EGCG) enhanced osteogenic differentiation of murine bone marrow mesenchymal stem cells by increasing the mRNA expression of osteogenesis-related genes, alkaline phosphatase activity and eventually mineralization. We further found EGCG supplementation preserved bone mass and microarchitecture in female rats during estrogen deficiency in the proximal tibia and lumbar spine at least in part by increasing bone morphogenetic protein-2 (BMP2). BMP2 can enhance de novo bone formation. Purpose: In this study, we evaluate the effect of local EGCG application in de novo bone formation in bone defect healing. Methods: Twenty-four rats aged 4 months were weight-matched and randomly allocated to 2 groups: defect control with vehicle treatment (control) and defect with 10 µM EGCG treatment (EGCG). Daily vehicle and EGCG were applied locally by percutaneous local injection 2 days after defect creation for 2 weeks. Four weeks after treatment, animals were sacrificed for micro-computed tomography (μ-CT) and biomechanical analysis. Results: Local EGCG at femoral defect can enhance de novo bone formation by increasing bone volume and subsequently improve mechanical properties including max load, break point, stiffness, area under the max load curve, area under the break point curve and ultimate stress. Conclusions: Local EGCG may enhance bone defect healing via at least partly by the de novo bone formation of BMP-2.

AB - Background: Previously, we found that (−)-epigallocatechin-3-gallate (EGCG) enhanced osteogenic differentiation of murine bone marrow mesenchymal stem cells by increasing the mRNA expression of osteogenesis-related genes, alkaline phosphatase activity and eventually mineralization. We further found EGCG supplementation preserved bone mass and microarchitecture in female rats during estrogen deficiency in the proximal tibia and lumbar spine at least in part by increasing bone morphogenetic protein-2 (BMP2). BMP2 can enhance de novo bone formation. Purpose: In this study, we evaluate the effect of local EGCG application in de novo bone formation in bone defect healing. Methods: Twenty-four rats aged 4 months were weight-matched and randomly allocated to 2 groups: defect control with vehicle treatment (control) and defect with 10 µM EGCG treatment (EGCG). Daily vehicle and EGCG were applied locally by percutaneous local injection 2 days after defect creation for 2 weeks. Four weeks after treatment, animals were sacrificed for micro-computed tomography (μ-CT) and biomechanical analysis. Results: Local EGCG at femoral defect can enhance de novo bone formation by increasing bone volume and subsequently improve mechanical properties including max load, break point, stiffness, area under the max load curve, area under the break point curve and ultimate stress. Conclusions: Local EGCG may enhance bone defect healing via at least partly by the de novo bone formation of BMP-2.

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