Epithelial-mesenchymal transition contributes to SWCNT-induced pulmonary fibrosis

Chih-Ching Chang, Mei-Ling Tsai, Hui Chun Huang, Chin Yu Chen, Shi Xun Dai

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

Previous studies suggest that single-walled carbon nanotube (SWCNT) exposure causes pulmonary fibrosis. We investigated the contribution of epithelial-mesenchymal transition (EMT) during SWCNT-induced pulmonary fibrosis. C57BL6 female mice were intratracheally instilled with SWCNT at 80 μg/mouse for up to 56 days. SWCNT exposure caused pulmonary epithelial and mesenchymal injury, followed by granulomatous and fibrotic changes. Immunofluorescence staining demonstrated the increasing occurrence of epithelial-derived fibroblasts up to 42 days post-exposure. Flow cytometry analysis revealed that 42.60% of N-cadherin (N-cad)-positive fibroblasts were derived from pulmonary epithelial cells, and, in separate experiments, 30.68% of SPC positive cells were stained for N-cad at 42 days. These epithelial-derived fibroblasts were functional in collagen production. With the progression of fibrosis, there were increases in the number of hyperplastic epithelial cells stained positively for TGF-β/p-Smad2 or β-catenin. Therefore, EMT contributes significantly to fibroblast expansion. Aberrant activations of TGF-β/p-Smad2 and β-catenin are postulated to induce EMT during SWCNT-induced pathogenic fibrosis.

Original languageEnglish
Pages (from-to)600-610
Number of pages11
JournalNanotoxicology
Volume6
Issue number6
DOIs
Publication statusPublished - 2012 Sep 1

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Carbon Nanotubes
Epithelial-Mesenchymal Transition
Pulmonary Fibrosis
Single-walled carbon nanotubes (SWCN)
Fibroblasts
Catenins
Fibrosis
Epithelial Cells
Lung
Flow cytometry
Cadherins
Collagen
Fluorescent Antibody Technique
Flow Cytometry
Chemical activation
Cells
Staining and Labeling
Wounds and Injuries
Experiments

All Science Journal Classification (ASJC) codes

  • Biomedical Engineering
  • Toxicology

Cite this

Chang, Chih-Ching ; Tsai, Mei-Ling ; Huang, Hui Chun ; Chen, Chin Yu ; Dai, Shi Xun. / Epithelial-mesenchymal transition contributes to SWCNT-induced pulmonary fibrosis. In: Nanotoxicology. 2012 ; Vol. 6, No. 6. pp. 600-610.
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Epithelial-mesenchymal transition contributes to SWCNT-induced pulmonary fibrosis. / Chang, Chih-Ching; Tsai, Mei-Ling; Huang, Hui Chun; Chen, Chin Yu; Dai, Shi Xun.

In: Nanotoxicology, Vol. 6, No. 6, 01.09.2012, p. 600-610.

Research output: Contribution to journalArticle

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