Equipped C1 chemical assimilation pathway in engineering Escherichia coli

Elevated carbon dioxide (CO2) level in the atmosphere leads to climate change. The use of fossil fuel is the most prevalent cause for the increase in CO2 emissions. Redirecting CO2 efficiently as a carbon source for the sustainable production of biofuels and commodity biochemicals is an urgent necessity. Microorganisms capable of CO2 sequestration or whose biological routes that improve the utilization of C1 chemical arouse intensive interest as they allow sustainable productions by C1 chemical instead of fuel. Nevertheless, significant contribution is required for the direct conversion of C1 chemical, especially from CO2 to value-added products, that would donate the economical competency. However, the progress of equipping CO2 assimilation pathway in Escherichia coli is rare. Thus, this chapter is mainly focused on the summary of CO2 assimilation based on the RuBisCo pathway in the engineered E. coli and provide detailed information of the analytical approaches, gene selection, and engineering strategies to reduce CO2 content toward a low-carbon society.