Escherichia coli K1 RS218 interacts with human brain microvascular endothelial cells via type 1 fimbria bacteria in the fimbriated state

Ching-Hao Teng, Mian Cai, Sooan Shin, Yi Xie, Kee Jun Kim, Ahmed Khan Naveed, Francescopaolo Di Cello, Sik Kim Kwang

Research output: Contribution to journalArticle

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Abstract

Escherichia coli K1 is a major gram-negative organism causing neonatal meningitis. E. coli K1 binding to and invasion of human brain microvascular endothelial cells (HBMEC) are a prerequisite for E. coli penetration into the central nervous system in vivo. In the present study, we showed using DNA microarray analysis that E. coli K1 associated with HBMEC expressed significantly higher levels of the fim genes compared to nonassociated bacteria. We also showed that E. coli K1 binding to and invasion of HBMEC were significantly decreased with its fimH deletion mutant and type 1 fimbria locked-off mutant, while they were significantly increased with its type 1 fimbria locked-on mutant. E. coli K1 strains associated with HBMEC were predominantly type 1 fimbria phase-on (i.e., fimbriated) bacteria. Taken together, we showed for the first time that type 1 fimbriae play an important role in E. coli K1 binding to and invasion of HBMEC and that type 1 fimbria phase-on E. coli is the major population interacting with HBMEC.

Original languageEnglish
Pages (from-to)2923-2931
Number of pages9
JournalInfection and Immunity
Volume73
Issue number5
DOIs
Publication statusPublished - 2005 May 1

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Endothelial Cells
Escherichia coli
Bacteria
Brain
Microarray Analysis
Oligonucleotide Array Sequence Analysis
Meningitis
Central Nervous System
Population
Genes

All Science Journal Classification (ASJC) codes

  • Parasitology
  • Microbiology
  • Immunology
  • Infectious Diseases

Cite this

Teng, Ching-Hao ; Cai, Mian ; Shin, Sooan ; Xie, Yi ; Kim, Kee Jun ; Naveed, Ahmed Khan ; Di Cello, Francescopaolo ; Kwang, Sik Kim. / Escherichia coli K1 RS218 interacts with human brain microvascular endothelial cells via type 1 fimbria bacteria in the fimbriated state. In: Infection and Immunity. 2005 ; Vol. 73, No. 5. pp. 2923-2931.
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Escherichia coli K1 RS218 interacts with human brain microvascular endothelial cells via type 1 fimbria bacteria in the fimbriated state. / Teng, Ching-Hao; Cai, Mian; Shin, Sooan; Xie, Yi; Kim, Kee Jun; Naveed, Ahmed Khan; Di Cello, Francescopaolo; Kwang, Sik Kim.

In: Infection and Immunity, Vol. 73, No. 5, 01.05.2005, p. 2923-2931.

Research output: Contribution to journalArticle

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AU - Kim, Kee Jun

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AU - Di Cello, Francescopaolo

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