Essential roles of basic helix-loop-helix transcription factors, Capsulin and Musculin, during craniofacial myogenesis of zebrafish

Gang-Hui Lee, Min Yen Chang, Chia Hao Hsu, Yau Hung Chen

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Capsulin and Musculin are basic helix-loophelix transcription factors, but their biophysiological roles in zebrafish cranial myogenesis are unclear. Expressions of endogenous capsulin transcripts are detected at the central-(∼24-hpf) and at dorsal-and ventral-mesoderm cores (∼30-72 hpf) of branchial arches. In contrast, musculin transcripts are expressed as a two-phase manner: early phase (20-22 hpf) expressions of musculin are detected at the head mesoderm, whereas late-phase (36-72 hpf) are detected at all presumptive head-muscle precursors. Knockdown of either capsulin or musculin leads to loss of all cranial muscles without affecting trunk muscle development. The defective phenotypes of Capsulin-and Musculin-morphant can be rescued by co-injection of mRNA of each other. Both myf5 and myod transcripts are down-regulated in the Capsulin-morphant while myod transcripts are up-regulated in the Musculin-morphant. Therefore, we propose a putative regulatory network to understand how capsulin/musculin regulate distinctly either myf5 or myod during zebrafish craniofacial myogenesis.

Original languageEnglish
Pages (from-to)4065-4078
Number of pages14
JournalCellular and Molecular Life Sciences
Volume68
Issue number24
DOIs
Publication statusPublished - 2011 Dec 1

Fingerprint

Basic Helix-Loop-Helix Transcription Factors
Muscle Development
Zebrafish
Mesoderm
Head
Branchial Region
Muscles
Transcription Factors
Phenotype
Messenger RNA
Injections

All Science Journal Classification (ASJC) codes

  • Molecular Medicine
  • Molecular Biology
  • Pharmacology
  • Cellular and Molecular Neuroscience
  • Cell Biology

Cite this

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abstract = "Capsulin and Musculin are basic helix-loophelix transcription factors, but their biophysiological roles in zebrafish cranial myogenesis are unclear. Expressions of endogenous capsulin transcripts are detected at the central-(∼24-hpf) and at dorsal-and ventral-mesoderm cores (∼30-72 hpf) of branchial arches. In contrast, musculin transcripts are expressed as a two-phase manner: early phase (20-22 hpf) expressions of musculin are detected at the head mesoderm, whereas late-phase (36-72 hpf) are detected at all presumptive head-muscle precursors. Knockdown of either capsulin or musculin leads to loss of all cranial muscles without affecting trunk muscle development. The defective phenotypes of Capsulin-and Musculin-morphant can be rescued by co-injection of mRNA of each other. Both myf5 and myod transcripts are down-regulated in the Capsulin-morphant while myod transcripts are up-regulated in the Musculin-morphant. Therefore, we propose a putative regulatory network to understand how capsulin/musculin regulate distinctly either myf5 or myod during zebrafish craniofacial myogenesis.",
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Essential roles of basic helix-loop-helix transcription factors, Capsulin and Musculin, during craniofacial myogenesis of zebrafish. / Lee, Gang-Hui; Chang, Min Yen; Hsu, Chia Hao; Chen, Yau Hung.

In: Cellular and Molecular Life Sciences, Vol. 68, No. 24, 01.12.2011, p. 4065-4078.

Research output: Contribution to journalArticle

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