Establishment and application of PCR-SSCP profile for molecular typing of Cordyceps fungi

Hsiao Che Kuo, Yong Lin Su, Huey Lang Yang, Tzong-Yueh Chen

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

A method for distinguishing isolates of the fungi belonging to genus Cordyceps, used as food/medicine in Eastern Asia, was developed. A PCR-single stranded conformation polymorphism-based profile was applied and established on the ITS2 region of rDNA for molecular typing in 48 different isolates of Cordyceps species. Compared with the DNA sequencing data and secondary structure prediction of single strand DNA, the method used was sensitive and could distinguish between two closely related species in the genus Cordyceps. This method could be very useful for confirming the identity of a specific strain of Cordyceps for researchers and food companies that produce the fermented Cordyceps products as a quality control method.

Original languageEnglish
Pages (from-to)311-325
Number of pages15
JournalFood Biotechnology
Volume22
Issue number4
DOIs
Publication statusPublished - 2008 Oct 1

Fingerprint

Cordyceps
Molecular Typing
Single-Stranded Conformational Polymorphism
Fungi
Polymerase Chain Reaction
fungi
Food
single-stranded conformational polymorphism
Far East
Ribosomal DNA
DNA Sequence Analysis
East Asia
Quality Control
quality control
food industry
control methods
medicine
sequence analysis
researchers
methodology

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Food Science
  • Applied Microbiology and Biotechnology

Cite this

Kuo, Hsiao Che ; Su, Yong Lin ; Yang, Huey Lang ; Chen, Tzong-Yueh. / Establishment and application of PCR-SSCP profile for molecular typing of Cordyceps fungi. In: Food Biotechnology. 2008 ; Vol. 22, No. 4. pp. 311-325.
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Establishment and application of PCR-SSCP profile for molecular typing of Cordyceps fungi. / Kuo, Hsiao Che; Su, Yong Lin; Yang, Huey Lang; Chen, Tzong-Yueh.

In: Food Biotechnology, Vol. 22, No. 4, 01.10.2008, p. 311-325.

Research output: Contribution to journalArticle

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