Establishment and characterization of a human-papillomavirus negative, p53-mutation negative human cervical cancer cell line

Cheng-Yang Chou, Yen Hui Chen, Ching Cherng Tzeng, Yueh Chin Cheng, Chin Feng Chang, Tzer Ming Chen

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

A human cervical cancer cell line, CX, was established from a patient with squamous cell carcinoma of the uterine cervix. The CX cells were epithelial in morphology with relatively large vesicular nuclei, and prominent nucleoli. Cytoplasmic organelles were generally sparse but tonofilaments were relatively abundant. The cells grew as a compact sheet with close membrane approximation interconnected by desmosome-like junctions. CX cells contained cytokeratin, but not vimentin. Elevated levels of squamous cell carcinoma antigen and carcinoembryonic antigen were detected in the cell supernatants. Population doubling time was estimated to be about 20 h. CX cells were not able to grow in soft agar and not tumorigenic in nude mice. Chromosome analysis revealed that CX cells were heterogeneous and mainly had a female diploid karyotype. Unlike cervical cancer cell lines published previously, CX cells were demonstrated to be human papillomavirus-negative, p53 mutation-negative, Based on the distinct characteristics, CX cell line may prove to be a useful tool for the study of human cervical carcinogenesis.

Original languageEnglish
Pages (from-to)173-181
Number of pages9
JournalCancer Letters
Volume102
Issue number1-2
DOIs
Publication statusPublished - 1996 Apr 19

Fingerprint

Uterine Cervical Neoplasms
Cell Line
Mutation
Desmosomes
Intermediate Filaments
Carcinoembryonic Antigen
Vimentin
Keratins
Diploidy
Karyotype
Nude Mice
Cervix Uteri
Organelles
Agar
Squamous Cell Carcinoma
Carcinogenesis
Chromosomes
Epithelial Cells
Membranes
Population

All Science Journal Classification (ASJC) codes

  • Oncology
  • Cancer Research

Cite this

Chou, Cheng-Yang ; Chen, Yen Hui ; Tzeng, Ching Cherng ; Cheng, Yueh Chin ; Chang, Chin Feng ; Chen, Tzer Ming. / Establishment and characterization of a human-papillomavirus negative, p53-mutation negative human cervical cancer cell line. In: Cancer Letters. 1996 ; Vol. 102, No. 1-2. pp. 173-181.
@article{0c26aa0038034a13b59d76f0633d4304,
title = "Establishment and characterization of a human-papillomavirus negative, p53-mutation negative human cervical cancer cell line",
abstract = "A human cervical cancer cell line, CX, was established from a patient with squamous cell carcinoma of the uterine cervix. The CX cells were epithelial in morphology with relatively large vesicular nuclei, and prominent nucleoli. Cytoplasmic organelles were generally sparse but tonofilaments were relatively abundant. The cells grew as a compact sheet with close membrane approximation interconnected by desmosome-like junctions. CX cells contained cytokeratin, but not vimentin. Elevated levels of squamous cell carcinoma antigen and carcinoembryonic antigen were detected in the cell supernatants. Population doubling time was estimated to be about 20 h. CX cells were not able to grow in soft agar and not tumorigenic in nude mice. Chromosome analysis revealed that CX cells were heterogeneous and mainly had a female diploid karyotype. Unlike cervical cancer cell lines published previously, CX cells were demonstrated to be human papillomavirus-negative, p53 mutation-negative, Based on the distinct characteristics, CX cell line may prove to be a useful tool for the study of human cervical carcinogenesis.",
author = "Cheng-Yang Chou and Chen, {Yen Hui} and Tzeng, {Ching Cherng} and Cheng, {Yueh Chin} and Chang, {Chin Feng} and Chen, {Tzer Ming}",
year = "1996",
month = "4",
day = "19",
doi = "10.1016/0304-3835(96)04157-2",
language = "English",
volume = "102",
pages = "173--181",
journal = "Cancer Letters",
issn = "0304-3835",
publisher = "Elsevier Ireland Ltd",
number = "1-2",

}

Establishment and characterization of a human-papillomavirus negative, p53-mutation negative human cervical cancer cell line. / Chou, Cheng-Yang; Chen, Yen Hui; Tzeng, Ching Cherng; Cheng, Yueh Chin; Chang, Chin Feng; Chen, Tzer Ming.

In: Cancer Letters, Vol. 102, No. 1-2, 19.04.1996, p. 173-181.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Establishment and characterization of a human-papillomavirus negative, p53-mutation negative human cervical cancer cell line

AU - Chou, Cheng-Yang

AU - Chen, Yen Hui

AU - Tzeng, Ching Cherng

AU - Cheng, Yueh Chin

AU - Chang, Chin Feng

AU - Chen, Tzer Ming

PY - 1996/4/19

Y1 - 1996/4/19

N2 - A human cervical cancer cell line, CX, was established from a patient with squamous cell carcinoma of the uterine cervix. The CX cells were epithelial in morphology with relatively large vesicular nuclei, and prominent nucleoli. Cytoplasmic organelles were generally sparse but tonofilaments were relatively abundant. The cells grew as a compact sheet with close membrane approximation interconnected by desmosome-like junctions. CX cells contained cytokeratin, but not vimentin. Elevated levels of squamous cell carcinoma antigen and carcinoembryonic antigen were detected in the cell supernatants. Population doubling time was estimated to be about 20 h. CX cells were not able to grow in soft agar and not tumorigenic in nude mice. Chromosome analysis revealed that CX cells were heterogeneous and mainly had a female diploid karyotype. Unlike cervical cancer cell lines published previously, CX cells were demonstrated to be human papillomavirus-negative, p53 mutation-negative, Based on the distinct characteristics, CX cell line may prove to be a useful tool for the study of human cervical carcinogenesis.

AB - A human cervical cancer cell line, CX, was established from a patient with squamous cell carcinoma of the uterine cervix. The CX cells were epithelial in morphology with relatively large vesicular nuclei, and prominent nucleoli. Cytoplasmic organelles were generally sparse but tonofilaments were relatively abundant. The cells grew as a compact sheet with close membrane approximation interconnected by desmosome-like junctions. CX cells contained cytokeratin, but not vimentin. Elevated levels of squamous cell carcinoma antigen and carcinoembryonic antigen were detected in the cell supernatants. Population doubling time was estimated to be about 20 h. CX cells were not able to grow in soft agar and not tumorigenic in nude mice. Chromosome analysis revealed that CX cells were heterogeneous and mainly had a female diploid karyotype. Unlike cervical cancer cell lines published previously, CX cells were demonstrated to be human papillomavirus-negative, p53 mutation-negative, Based on the distinct characteristics, CX cell line may prove to be a useful tool for the study of human cervical carcinogenesis.

UR - http://www.scopus.com/inward/record.url?scp=0029984706&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0029984706&partnerID=8YFLogxK

U2 - 10.1016/0304-3835(96)04157-2

DO - 10.1016/0304-3835(96)04157-2

M3 - Article

VL - 102

SP - 173

EP - 181

JO - Cancer Letters

JF - Cancer Letters

SN - 0304-3835

IS - 1-2

ER -